Elucidation of molecular evolutinary mechanisms of HIV and HCV

阐明 HIV 和 HCV 的分子进化机制

• 批准号: 07404043
• 负责人: GOJOBORI Takashi
• 金额: $ 19.01万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07404043/
• 关键词: HIV; hepatitis C virus; molecular evolution; hepatitis B virus; GB virus; phylogenetic tree; subtype; nucleotide substitution; 塩基置換

The main purpose of this research project is to elucidate the evolutionary mechanisms of evolutionary processes of HIV (Human Immunodeficiency Virus) and HCV (Hepatitis C Virus). To accomplish this purpose, we conducted the research and obtained the following results : (1) We successfully constructed the databases for HIV and HCV spearately. In particular, the CD-ROM version of the HCV database has been distributed to all participants of the international HCV workshop at Kyoto. Moreover, the international meeting of the HCV nomenclature committee hosted by CDC,which was held at Sanata Fe in the United States in 1997, decided to adopt our HCV database as an official HCV database. (2) We successfully constructed phylogenetic trees for HIV clones isolated from single patients. It suggested that this type of analysis was very useful for elucidatinig the generating mechanisms of viral variants. (3) It also showed that the analysis of intra-body evolution of viral clones gave an insight into the effect of anti-viral drugs on the generation of viral variation. (4) We examined the infection routes such as needle stick accidents and husband-and-wife relationships from the viewpoint of molecular evolution. In particular, the frequency of infection through the husband and wife relationship was found to be very low.

该研究项目的主要目的是阐明HIV（人类免疫缺陷病毒）和HCV（丙型肝炎病毒）进化过程的进化机制。为了实现这一目的，我们进行了研究并取得了以下成果：（1）成功地分别构建了HIV和HCV数据库。特别是，HCV 数据库的 CD-ROM 版本已分发给京都国际 HCV 研讨会的所有参与者。此外，1997年在美国Sanata Fe召开的由CDC主办的HCV命名委员会国际会议决定采用我们的HCV数据库作为官方HCV数据库。 (2)我们成功构建了从单个患者分离的HIV克隆的系统发育树。这表明这种类型的分析对于阐明病毒变异体的产生机制非常有用。 (3)还表明，对病毒克隆体内进化的分析可以深入了解抗病毒药物对病毒变异产生的影响。 （4）从分子进化的角度考察了针刺事故、夫妻关系等感染途径。特别是通过夫妻关系感染的频率非常低。

项目成果

Okamoto Y.: "Father-to-mother・to-infant transmission of HIV-1;clonally transmitted isolate of infant mutates more rapidly than that of the mother and rapidly loses reactivity with neutralizing antibody." Microbi ol Immun 01. 41. 131-138 (1997)

Okamoto Y.：“HIV-1 的父亲到母亲·婴儿传播；克隆传播的婴儿分离株比母亲的变异更快，并且迅速失去与微生物免疫 01. 41. 131 的反应性。” -138 (1997)

Shiina, T., A.Ando, T.Imanishi, H.Kawata, K.Hanzawa T.Gojobori, H.Inoko, and S.Watanabe: ""Isolation and characterization of cDNA clones for Japanese quail (Coturnix japonica) major histocompatibility complex (MhcCoja) class I molecules."" Immunogenetics.

Shiina，T.，A.Ando，T.Imanishi，H.Kawata，K.Hanzawa T.Gojobori，H.Inoko 和 S.Watanabe：“日本鹌鹑 (Coturnix japonica) 主要组织相容性 cDNA 克隆的分离和表征

Okamoto Y., K.Shiosaki, Y.Eda, S.Tokiyoshi, Y.Yamaguchi, T.Gojobori, T.Hachimori, S.Yamazaki, and M.Honda: ""Father-to-mother-to-infant transmission of HIV-1 : clonally transmitted isolate of infant mutates more rapidly than that of the mother and rapidly

Okamoto Y.、K.Shiosaki、Y.Eda、S.Tokiyoshi、Y.Yamaguchi、T.Gojobori、T.Hachimori、S.Yamazaki 和 M.Honda：“”从父亲到母亲到婴儿的传播

Suzuki, Y., and T.Gojobori: ""The origin and evolution of ebola and marburg viruses."" Mol.Biol.Evol.14(8). 800-806 (1997)

Suzuki, Y. 和 T.Gojobori：“埃博拉病毒和马尔堡病毒的起源和进化。”Mol.Biol.Evol.14(8)。

Shiga, H., Y.Shioda, H.Tomiyama, Y.Takamiya, S.Oka, S.Kimura, Y.Yamaguchi, T.Gojobori, Rammensee, K.Miwa, and M.Takiguchi: ""Identificaton of multiple HIV-1 cytotoxic T-cell epitopes presented by human leukocyte antigen B35 molecules."" AIDS. 10. 1075-108

Shiga，H.，Y.Shioda，H.Tomiyama，Y.Takamiya，S.Oka，S.Kimura，Y.Yamaguchi，T.Gojobori，Rammensee，K.Miwa 和​​ M.Takiguchi：“”多种 HIV 的鉴定