Prediction of gene functions by use of molecular evolutionary approach

利用分子进化方法预测基因功能

• 批准号: 07504009
• 负责人: GOJOBORI Takashi
• 金额: $ 13.12万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07504009/
• 关键词: molecular evolution; prediction of gene function; functional domain; Molecular Phylogenetic Tree; serine protease; MHC (major histocompatibility complex); nucleotide sequence; sequencing; セリンプロテアーゼ; molecular evolution; prediction of gene function; functional domain; Molecular Phylogenetic Tree; serine protease; MHC (major histocompatibility complex); nucleotide sequence; sequencing; セリンプロテアーゼ

The main purpose of the present research project is to extract functional domains from the nucleotide sequences of homologous gene groups by use of molecular evolutionary approach. To attain this purpose, we first developed the the new method for constructing phylogenetic trees based upon the minimum description length principle. By using this new method, we identified several functional domains of serine protease genes as an example. Because this method was so successful, weextended our approach to all homologous gene groups. Then, we constructed the 'so-called' sequence motif database, where sequence motif represents characteristics of the sequence specific to a particular function. Moreover, we produced nucleotide sequences of genes whose data availability is sos limited by conducting the PCR experiments. In order to establish the sequence techniques in our laboratory, we practiced the PCR experiment by using fish mitochondrial DNAs. These results obtained in this research project is significant for identifying and predicting the gene functions from the nucleotide sepuence data.

本研究项目的主要目的是通过使用分子进化方法从同源基因组的核苷酸序列中提取功能结构域。为了实现这一目的，我们首先根据最小描述长度原理开发了构造系统发育树的新方法。通过使用这种新方法，我们将丝氨酸蛋白酶基因的几个功能域视为一个例子。由于这种方法是如此成功，因此我们将我们的方法推迟到所有同源基因组。然后，我们构建了“所谓的”序列基序数据库，其中序列基序代表特定函数的序列的特征。此外，我们产生了基因的核苷酸序列，其数据可用性通过进行PCR实验而受到限制。为了在我们的实验室中建立序列技术，我们通过使用FISH线粒体DNA进行了PCR实验。在该研究项目中获得的这些结果对于鉴定和预测核苷酸链球菌数据的基因功能非常重要。