Gene expressions of Cross-talking of transcription factors controlling development of oral-maxillofacial region

控制口颌面部发育的转录因子串扰的基因表达

基本信息

批准号：
14571866
负责人：
SATO Yoshinori
金额：
$ 2.24万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571866/
关键词：
Oral-maxillofacial region BMP CBFA-1 mandible maxilla 骨形成 RNAKL OGF 軟骨顎顔面の発育 mRNA Meckel軟骨

项目摘要

Development of oral-maxillofacial region is controlled by various growth factors.Recently, core binding protein-1 (CBFA-1) was shown to be a critical transcriptional regulator of osteoblasts differentiation, Mutation in this gene causes cleidocranial dysplasia. CBFA-1 is deeply involved in formation of development of oral-maxillofacial region and tooth formation. Bone morphogenetic proteins (BMPs) were originally identified as proteins that induce ectopic bone formation. More recent studies have shown that BMPs are a multifunctional growth factor that regulates cell proliferation and the synthesis of extracellular matrices and acts as a signal in epithelial-mesenchymal interaction during tooth development. In the recent studies, it was demonstrated that gene expression of CBFA-1 is induced by BMP. Therefore,. localizations and gene expressions of BMP and CBFA-1 were investigated in mouse embryos.The distributions of mRNA and localization were co-localized. The expressions CBFA-1 mRNA w … More ere detected in the mesenchymal condensations of front nasal regions of skull at E12 days. The gene expressions of these regions were spread from E13 and E14 days. Gene expressions CBFA-1 were detected in the osteoblasts along the Meckel' s cartilage. From 0 to 21 days the strong gene expressions were detected in the osteoblasts of maxilla and mandible.In the tooth germs of the bud stage, gene expressions were localized in the tooth buds and the mesenchymal cells of the dental follicle. In the cup stage, strong expressions were detected in the enamel organ and the primary enamel knots. Gene expressions were trans-localized to the dental papilla and dental follicle. The secondary enamel knots showed strong gene expression.The distributions of mRNA and localization of BMP-4 were co-localized with CBFA-1. During bone formation, BMP-4 was localized in the preosteoblasts.These data show that BMP-4 was related in the early differentiation of osteoblasts but CBFA-1 related maturation of osteoblasts. Less

口腔颌面部的发育受多种生长因子的控制。最近，核心结合蛋白1（CBFA-1）被证明是成骨细胞分化的关键转录调节因子，该基因的突变导致锁骨颅骨发育不良。参与口腔颌面区域发育和牙齿形成的骨形态发生蛋白（BMP）最初被鉴定为诱导异位骨形成的蛋白质。研究表明，BMP 是一种多功能生长因子，可调节细胞增殖和细胞外基质的合成，并在牙齿发育过程中充当上皮间质相互作用的信号，在最近的研究中，BMP 诱导了 CBFA-1 的基因表达。因此，研究了BMP和CBFA-1在小鼠胚胎中的定位和基因表达。CBFA-1 mRNA的表达和定位是共定位的。 …更多在 E12 天的头骨前鼻区域的间质凝结中检测到这些区域的基因表达从 E13 和 E14 天开始在沿梅克尔软骨的成骨细胞中检测到基因表达。 21天时，在上颌骨和下颌骨的成骨细胞中检测到较强的基因表达。在芽期牙胚中，基因表达定位于牙芽和牙囊间充质细胞，在牙釉质器官和初级牙釉质结中检测到强表达，基因表达反式定位至牙乳头和牙囊。牙釉质结表现出较强的基因表达。BMP-4的mRNA分布和定位与CBFA-1共定位。在骨形成过程中，BMP-4定位于牙釉质结。这些数据表明，BMP-4与成骨细胞的早期分化相关，但CBFA-1与成骨细胞的成熟相关较少。