Molecular structure in regeneration of maxilla, mandible and periodontal tissue using recombinat BMP

使用重组 BMP 再生上颌骨、下颌骨和牙周组织的分子结构

• 批准号: 13672125
• 负责人: TANAKA Hiroshi
• 金额: $ 2.3万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672125/
• 关键词: Bone induction; BMP; TGE-beta; CBFA-1; Smad; CBFA-1; 骨再生; mRNA; Noggin

Bone transplantation is generally performed by treatment for bone defection : Recently, regeneration theraputics using growth factors has been introduced. BMPs play a fundamental role in bone formation and bone regeneration. The bone induction using recombinant BMP was investigated.Materials and Method1. BMP and HAP mixtureThe mixture of decalcified bone matrix including 1 mg of recombinant BMP were embedded in subcutaneous tissue.2. Tissue engineering methodThe bone stroma cells isolated from the bone marrow, then these cells were cultured with HAP or calcium phosphate cement CPC with BMP. These cell-CPC complex were embedded in the ubcutaneous tissue.Results1. BMP and HAP mixtureCartligious tissue formed at 5days after the implant then bone tissue was formed at 10 days after implantation.2. Tissue engineering methodThe cell-CPC complex showed stronger bone induction than that of cell-HAP complex. After 2 week of implantation of cell-CPC, thick layer of connective tissue was covered on implants. After 4 week the layer was calcified. Hence, in the implantation of the connective tissue layer was formed at 4 weeks after the implantation. TGF-β super family was expressed in the preosteoblasts, fibroblasts and osteoclasts around implants. These data suggests that TGF-β super family plays central role in ectopic bone formation.

骨移植是通过治疗骨骼缺陷的一般性：使用生长培训的再生治疗中的骨骼在骨形成和骨骼再生中起着基本作用。包括1毫克重组BMP在皮下组织中嵌入在植入后10天形成。2。结缔组织层是在植入TGF-β超级Fas后4周形成的，在异位骨形成中表达的中心作用。

项目成果

稲毛稔彦, 他: "歯胚の成長に伴う硬組織形成の微細構造"日本大学歯学部研究紀要(一般教育). 29号. 45-53 (2001)

Toshihiko Inage 等人：“与牙胚生长相关的硬组织形成的精细结构”日本大学牙科学院通报（普通教育）29, 45-53 (2001)。

稲毛稔彦, 大井田新一郎, 他: "抜歯窩の治癒過程におけるBMPの遺伝子発現"日本大学歯学部総合歯学研究所研究報告書. 03号. 101-111 (2003)

Toshihiko Inage、Shinichiro Oida等人：“拔牙槽愈合过程中BMP基因的表达”日本大学牙科学院牙科研究所03号101-111(2003)。

稲毛稔彦, 後藤 實, 大井田新一郎他: "マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について"日大歯学. 77・2. 141-149 (2003)

Toshihiko Inage、Minoru Goto、Shinichiro Oida 等：“CBFA-1 和 BMP-4 在小鼠颌面发育中的定位”日本大学牙科 77・2（2003 年）。

稲毛稔彦: "歯科におけるBMPと骨誘導の発展性"日本歯科医師会雑誌. 53.11. 1029-1036 (2001)

Toshihiko Inage：“牙科中 BMP 和骨诱导的发展”，日本牙科协会杂志 53.11（2001 年）。

稲毛稔彦, 他: "電子顕微鏡を用いたエナメル芽細胞におけるamelogenin mRNAの遺伝子発現"日大歯学. 76・5. 491-499 (2001)

Toshihiko Inage等人：“使用电子显微镜观察成釉细胞中牙釉蛋白mRNA的基因表达”日本大学牙科76·5（2001）。