Gene expressions of growth factor cross-talking in tissue enginearing used cells stimulated by BMP

BMP刺激的组织工程用细胞中生长因子串扰的基因表达

• 批准号: 14571917
• 负责人: SEKIWA Tadanobu
• 金额: $ 1.86万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571917/
• 关键词: Tissue engineering; BMP; Growth factor; Bone induction; mRNA; TGF-β; RANKL; OPG; CBFA-1; Smad

Recently, therapeutics using growth factors and extracellular matrix such as EMDOGAIN or BMP has been introduced dental practice : The growth factors therapeutics need a large amount of proteins and the mechanisms of healing are still obscure. In the present study, bone marrow. stroma cells and HAP were used for bone formation of tissue engineering.Materials and MethodsWister rats weighting 100g were used for this study. The stroma cells of bone marrow were isolated from bone marrow by aspiration of syringe. Stroma cells were cultured in alpha-MEM medium with HAP or CPC (calcium phosphate cement) by adding BMP. Stroma cells were cultured for 1 week. Then, cultured cells-HAP complex were transplanted in the artificial hole of calvaria.ResultsThe cell-CPC complex showed stronger bone formation than that of cell-HAP complex. After 2 week of implantation of cell-CPC, thick layer of connective tissue was covered on implants. After 4 week the layer was calcified. Hence, in the implantation of the connective tissue layer was formed at 4 weeks after the implantation. TGF-bet a superfamily was expressed in the preosteoblasts, fibroblasts and osteoclasts around implants. These data suggests that TGF-beta superfamily plays central role in ectopic bone formation.

最近，已经引入了使用生长因子和细胞外基质的治疗剂，例如emdogain或bmp：牙科实践：生长因子治疗需要大量蛋白质，并且愈合的机制仍然晦涩难懂。在本研究中，骨髓。基质细胞和HAP用于组织工程的骨骼形成。材料和方法代表大鼠加权100G。通过注射器的抽吸从骨髓中分离出骨髓的基质细胞。通过添加BMP，在具有HAP或CPC（磷酸钙水泥）的Alpha-MEM培养基中培养基质细胞。将基质细胞培养1周。然后，将培养的细胞-MAP复合物移植在瓦尔瓦里亚的人造孔中。分子细胞-CPC复合物的骨形成比细胞-AP复合物的骨形成更强。植入细胞CPC 2周后，植入物覆盖了厚的结缔组织层。 4周后，将层钙化。因此，在植入后4周形成结缔组织层的植入中。 TGF-bet在植入物周围的骨细胞，成纤维细胞和破骨细胞中表达了一个超家族。这些数据表明，TGF-Beta超家族在异位骨形成中起着核心作用。

项目成果

稲毛稔彦, 関和忠信, 大井田新一郎他: "マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について"日大歯学. (発表予定).

Toshihiko Inage、Tadanobu Sekiwa、Shinichiro Oida 等人：“CBFA-1 和 BMP-4 在小鼠颌面发育中的定位”日本大学牙科学院（待提交）。

稲毛稔彦, 寺門正昭他8名: "電子顕微鏡を用いたエナメル芽細胞におけるamelogenin mRNAの発現"日大歯学. 76. 491-499 (2002)

Toshihiko Inage、Masaaki Terakado 等 8 人：“使用电子显微镜在成釉细胞中表达釉原蛋白”日本大学牙科 76. 491-499 (2002)。

稲毛稔彦 他: "歯周組織とエナメルタンパク"永末書店. 61-69 (2002)

Toshihiko Inage 等人：“牙周组织和牙釉质蛋白”，Nag​​asue Shoten 61-69 (2002)。

稲毛稔彦, 他: "歯胚の成長に伴う硬組織形成の微細構造"日本大学歯学部研究紀要(一般教育). 29号. 45-53 (2001)

Toshihiko Inage 等人：“与牙胚生长相关的硬组织形成的精细结构”日本大学牙科学院通报（普通教育）29, 45-53 (2001)。

稲毛稔彦, 寺門正昭他15名: "マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について"日大歯学. 77. 141-149 (2003)

Toshihiko Inage、Masaaki Terakado 等 15 人：“CBFA-1 和 BMP-4 在小鼠颌面发育中的定位”日本大学牙科 77. 141-149 (2003)。