The cross talking of the gene and regeneration in regeneration of periodontal tissue - main role of CBFA-1 -

牙周组织再生中基因与再生的交叉对话-CBFA-1的主要作用-

• 批准号: 15592141
• 负责人: TANAKA Hiroshi
• 金额: $ 2.18万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592141/
• 关键词: CBFA-1; CDF; BMP; root formation; odontoblast; Hertwig上皮鞘; GDF; Runz2

Deficiency of Core binding factor A-1(CBFA-1) causes cleidocranial disostosis and play important role differentiation of the osteoblasts precursor cells to the osteoblast. CBFA-1 is expressed in many tissues and controls differentiation and growth of cells. Expression of CBFA-1 mRNA is regulated by the expression of BMP.In the present study, cross talking of BMP and CBFA-1 mRNA were studied by in situ hybridization and immunohistochemistry.Under sodium pentobarbital anesthesia, Wistar rats (40 g) were perfused via the left ventricles with 4% paraformaldehyde solution diluted in 0.025 M phosphatebuffer (pH 7.4). The embryos were demineralized with 0.1M EDTA (pH 7.4), and after dehydration in ethanol tissue, they were embedded in paraffin and then cut longitudinally into 5 μm thick slices along the long axis of incisors.These animal experiments were approved by the Institutional Ethical Committee for Animal Experiments and properly carried out under the control of the Guidelines for Animal Experimentation in Nihon University School of Dentistry.During the development of oralmaxillo-facial region, expression of BMP was appeared in preosteoblasts of the region of the bone and tooth formation. Expression of CBFA-1 mRNA was detected slightly later stage than those of BMP mRNA. CBFA-1 mRNA was expressed in the osteoblasts and osteocytes. GDF was strongly expressed in the cementoblasts.During root formation, BMP mRNA was expressed in the cells of epithelial root sheath of Hertwig (ERSH). On the other hand, CBFA-1 mRNA was expressed in the dental palilla cells and preodontoblasts facing the ERSH.Expression of BMP stimulates transcription level of CBFA-1 mRNA. Therefore, the expression of BMP from the ERSH induce the expression of CBFA-1 of n the dental palilla cells and preodontoblasts facing the ERSH. The cross talking of BMP and CBF-1 regulate the differentiation of the cells forming root formation and bone.

核心结合因子A-1（CBFA-1）的缺乏症，在许多组织中表达了成骨细胞的成骨细胞与成骨细胞的重要作用。在本研究中，由BMP的表达进行了定期，通过原位杂交和杂交进行了对BMP和CBFA-1 mRNA的交叉谈论。五脑巴比妥钠在pentobarbital麻醉下，Wistar大鼠（40 g）通过左侧的4次灌注4在0.025 M磷酸盐中稀释的多聚甲醛（pH 7.4）。在NIHON大学的实验指南中，实验实验的动物在骨骼和MP mRNA中表达了BMP的表达。成骨细胞和骨细胞。 n牙prilla细胞的CBFA-1表达和前牙细胞的表达，fa fa fa the ersh。

项目成果

マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について

CBFA-1和BMP-4在小鼠颌面部发育中的定位

• 发表时间: 2003
• 期刊: 日大歯学 77
• 作者: 稲毛稔彦;田中博;他15名
• 通讯作者: 他15名

Localization of CBFA-1 and BMP-4 during Oral Maxillofaciai Development in Mice.

CBFA-1 和 BMP-4 在小鼠口腔颌面发育过程中的定位。

• 发表时间: 2003
• 期刊: Nihon University Dental journal Vol.77-2
• 作者: Toshihiko Inage;Hiroshi Tanaka;Shinichiro Oida;et al.
• 通讯作者: et al.

Localization Of CBFA-1/Runx2 in Ameloblasls.

CBFA-1/Runx2 在成釉细胞中的定位。

• 期刊: Nihon University School of Dentistry Research Bulletin Vol.31
• 作者: Fumiyuki Kuwata;Toshihiko Inage;et al.
• 通讯作者: et al.

エナメル芽細胞におけるCBFA-1/Runx2の局在

CBFA-1/Runx2 在成釉细胞中的定位

• 发表时间: 2003
• 期刊: 日本大学歯学部研究紀要 31
• 作者: 桑田文幸;稲毛稔彦;他3名
• 通讯作者: 他3名