Detection of unstable atheroma plague by contrast ultrasound imaging

超声造影检测不稳定粥样斑块

基本信息

批准号：
17590741
负责人：
OHMORI Koji
金额：
$ 2.24万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Activity of atheroma plaque depends on the severity of ongoing inflammation. Therefore, we applied contrast ultrasound to inflammation imaging. We obtained images of kidneys by diagnostic ultrasound pressure after suspension of ultrasound emission for 10 minutes allowing phagocytosis of microbubbles (MB) by leukocytes following MB injection. By subtracting perfusion image from this image, we extracted signals from retained (phagocytosed) MB. The signal distributed in the medulla in the ischemia reperfusion model and in the cortex in the glomerulonephritis model, which well agreed with the distribution of leukocytes in respective models. We applied this imaging method to mouse hindlimbs ischemia model of hypoperfused-inflamed tissue that better mimics atheroma plaque. The similar settings provided an opacification that represents retained MB. Thus, using ordinary acoustic pressure we can image retained microbubbles in the inflamed tissue by devising timing. However, histological assessm … More ent of its safety revealed that leukocyte infiltration was aggravated after 24 hours of imaging, which was unfavorable side effect potentially causing plaque rupture. In order to avoid this side effect a low-acoustic pressure apparatus with advanced sensitivity needs to be devised in the future. However, the side effect causing leukocyte infiltration could be applicable to therapeutic angiogenesis, because leukocytes release vascular endothelial growth factor and granulocyte colony stimulating factor (G-SF) has been used in therapeutic angiogenesis. We applied this method to the same model and found that it significantly further augmented angiogenesis in combination with pre-administration of 300 microgram G-CSF which was minimum effective dose at monotherapy. Thus, inflammation imaging by contrast ultrasound using current apparatus can evaluate leukocyte infiltration or activity of inflammation which determine the severity of respective pathology, but aggravated inflammation. However, this side effect was applicable to enhancement of therapeutic angiogenesis which would be worth while optimizing in the future study. Less

动脉瘤菌斑的活性取决于持续注射的严重程度。因此，我们将对比度超声施加到炎症成像中。我们通过诊断超声发射后通过诊断超声压力获得了肾脏图像，持续10分钟，在MB注射后通过白细胞吞噬微泡（MB）。通过从此图像中减去灌注图像，我们从保留（吞噬）MB中提取信号。在缺血再灌注模型和肾小球肾炎模型中的皮质中分布在髓质中的信号，该模型与相对模型中白细胞的分布非常一致。我们将这种成像方法应用于小鼠后肢缺血模型的垂体爆发组织，以更好地模仿动脉粥样硬化斑块。类似的设置提供了代表保留MB的不透明化。这是使用普通的声压力，我们可以通过设计时间来对发炎组织中保留的微泡形象。然而，组织学评估……更多的安全性显示，成像24小时后，白细胞浸润是汇总的，这是不利的副作用，可能导致斑块破裂。为了避免这种副作用，将来需要设计具有高级灵敏度的低声压设备。但是，引起白细胞浸润的副作用可能适用于治疗性血管生成，因为白细胞释放血管内皮生长因子和粒细胞菌落刺激因子（G-SF）已用于治疗性血管生成。我们将此方法应用于同一模型，发现它与300微克G-CSF的预先管理相结合，从而显着增强了血管生成，这在单一疗法时是最小有效剂量。这是通过当前设备对相反超声进行的炎症成像可以评估白细胞浸润或感染的活性，这决定了相对病理的严重程度，但炎症的炎症。但是，这种副作用适用于增强治疗性血管生成的作用，这在未来的研究中优化时值得。较少的

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

微气泡的最新技术，从微气泡和纳米气泡的产生和特性到在食品、农业、环境净化和医学中的应用。

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
河野雅和;大森浩二(共著)他
通讯作者：
大森浩二(共著)他

Angiogenesis using ultrasonic microbubble destruction Induction of vascular endothelial growth factor by microhemorrhage Assessment of its safety and efficacy.(Review article in Japanese)

使用超声微泡破坏血管生成通过微出血诱导血管内皮生长因子评估其安全性和有效性。（日语评论文章）

DOI：
发表时间：
2005
期刊：
Ultrasound TECHNO 17
影响因子：
0
作者：
Fukuyama K;Ichiki T;Imayama I;Ohtsubo H;Ono H;Hashiguchi Y;Takeshita A;Sunagawa K.;Maruyama R;Yokoyama M;Ono H et al.;Ohmori K
通讯作者：
Ohmori K

Pre-administration of G-CSF potentiates therapeutic angiogenesis by ultrasonic microbubble destruction

预施用 G-CSF 通过超声微泡破坏增强治疗性血管生成