Transcriptional regulation of the human ABO histo-blood group genes is dependent on the negative regulation through the N box upstream of the proximal promoter

人类 ABO 组织血型基因的转录调控依赖于近端启动子上游 N 盒的负调控

• 批准号: 15590575
• 负责人: KOMINATO Yoshihiko
• 金额: $ 1.86万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590575/
• 关键词: ABO gene; transcription; N box; repression; promoter; ABO遺伝子

Our previous studies of the transcriptional regulatory mechanism of the human ABO gene indicated that negative regulatory elements are present in the sequence upstream of the proximal promoter, suggesting that transcription from the ABO proximal promoter is in part controlled by silencer elements just upstream of the promoter. In this study, we have confirmed that ABO gene expression is negatively regulated by the sequence upstream of the proximal promoter in KATOIII cells, and that the region between -202 and -118 is involved in the negative regulation in cells of both epithelial and erythroid lineages. Transient transfection experiments in KATOIII cells using a luciferase reporter plasmid carrying mutated N box at -196 to -191 demonstrated that the N box is a negative regulatory element in the -202 to -118 sequence. Using electrophoretic mobility shift assay, we showed that the N box binds with a nuclear factor, termed RACP, derived from KATOIII cells. These results suggest that repression of transcription from the ABO proximal promoter is partially dependent upon the N box, and that down-regulation of RACP may relieve the repression, thereby leading to expression of the ABO gene during maturation of cells in the epithelial lineage as well as the erythroid lineage.

我们先前对人ABO基因转录调控机制的研究表明，近端启动子上游的序列中存在负调节元件，这表明ABO近端启动子的转录部分由启动子上游的Silencer元素控制。在这项研究中，我们已经证实，ABO基因表达受Katoiii细胞近端启动子上游的序列负调节，并且-202和-118之间的区域参与上皮和红细胞谱系的细胞负调节。使用荧光素酶报告基质粒在-196至-191中带有突变的N盒的荧光素酶报告基质粒在KatoIII细胞中进行的瞬时转染实验表明，N框是-202至-118序列中的负调节元件。使用电泳迁移率转移测定法，我们表明N盒与源自katoiii细胞的RACP结合。这些结果表明，抑制ABO近端启动子的转录部分取决于N框，RACP的下调可能会缓解抑制作用，从而导致上皮谱系中细胞成熟期间表达ABO基因的表达以及红细胞。

项目成果

Yamamoto M, Yamamoto F, Luong TT, Williams T, Kominato Y, Yamamoto F: "Expression profiling of 68 glycosyltransferase genes in 27 different human tissues, by the Systematic Multiplex RT-PCR (SMRT-PCR) method, revealed clustering of sexually related tissue

Yamamoto M、Yamamoto F、Luong TT、Williams T、Kominato Y、Yamamoto F：“通过系统多重 RT-PCR (SMRT-PCR) 方法对 27 个不同人体组织中的 68 个糖基转移酶基因进行表达谱分析，揭示了性相关的聚类

Transcriptional regulation of the human ABO histo-blood group genes is dependent on the N box upstream of the proximal promoter

• DOI: 10.1111/j.0041-1132.2004.04028.x
• 发表时间: 2004-12-01
• 期刊: TRANSFUSION
• 影响因子: 2.9
• 作者: Kominato, Y;Hata, Y;Kishi, K
• 通讯作者: Kishi, K

Molecular evolution of shark and other vertebrate DNases I

鲨鱼和其他脊椎动物 DNA 酶 I 的分子进化

• 发表时间: 2004
• 期刊: European Journal of Biochemistry 271・22
• 作者: Kakinoki K;Fujino Y;Suzuki Y;Li S;Tanioka Y;Sakai T;Kuroda Y;Cesar F.Ortega-Cava;Masanori Abe;Komine M;Toshihiro Yasuda
• 通讯作者: Toshihiro Yasuda

Transcriptional regulation of the human ABO histo-blood group genes in dependent on the N box upstream of the proximal promoter

人类 ABO 组织血型基因的转录调控依赖于近端启动子上游的 N 盒

• 发表时间: 2004
• 期刊: Transfusion 44
• 作者: Kominato Y;Hata Y;Matsui K;Takizawa H;Tsukada J;Nakajima T;Kaneko Y;Kishi K.
• 通讯作者: Kishi K.

A case of postoperative hemorrhage after a hysterectomy in which a bleeding point of the left uterine artery was identified by postmortem angiography

子宫切除术后出血一例，尸检血管造影发现左子宫动脉出血点

• 发表时间: 2004
• 期刊: Legal Medicine 6・3
• 作者: Goto T;Tanioka Y;Sakai T;Matsumoto I;Kakinoki K;Tanaka T;Li S;Yoshikawa T;Fujino Y;Suzuki Y;Kuroa Y;Yoshihiko Kominato
• 通讯作者: Yoshihiko Kominato