Studies on the evaluation and development of Colombian Medicinal Plants

哥伦比亚药用植物评价与开发研究

基本信息

批准号：
12576030
负责人：
FUJIMOTO Yoshinori
金额：
$ 9.6万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12576030/
关键词：
International Exchange of Researchers Colombia Medicinal Plant Natural product chemistry Bioactive substances 生物活性 Mikania glomerata Hydrocotyle umbellata Campomanesia lineatifolia Inga spectabilis Niphogeton ternata Sicana odorifera

项目摘要

During the term of project we visited Colombia three times and stepped into local markets in Bogota, suburb areas of Bogota and Leticia in Colombian Amazon, and obtained a number of information on folk medicinal plants and prepared methanol extract samples of 36 medicinal plants for evaluations. In the screening of brine shrimp lethality bioassay, the extracts of Spondias purpurea seeds, Anacardium giganteum seeds and Mikania glomerata showed the toxic activity. In the evaluation of anti-oxidant activity monitored by DPPH radical scavenging effects, leave extracts of Inga spectabilis and Campomanesia lineatifolia displayed potent activity. In the assay of the inhibition of HCV RNA polymerase, extracts of Cedrela odorata burk and Anacardium giganteum seeds found to be effective. In the search of synergistic effect of plant extracts to enhance the action of antibiotics against MRSA, the extracts of Matayba elegans leaves/stem, Campsiandra commosa burk and Cupania americana aerial part exhibited significant activity. Bioassay-guided separation of the extract allowed us to characterize the some of the active constituents. Among these medicinal plant extracts we have isolated and determined the structures of 15 new substances.

在项目期限期间，我们访问了哥伦比亚三次，进入了波哥大的当地市场，波哥大的郊区和洛杉矶亚马逊的莱蒂西亚，并获得了有关民间药用植物的许多信息，并准备了36种药用植物的甲醇提取物样品进行评估。在筛选盐水虾致死性生物测定中，紫红葡萄氏蛋白酶的提取物，giganteum seeds和mikania glomerata的提取物显示出毒性活性。在通过DPPH自由基清除效应监测的抗氧化活性的评估中，留下了Inga Spectabilis和Campomanesia lineatifolia的提取物表现出强大的活性。在抑制HCV RNA聚合酶的测定中，发现有效的Cedrela odorata Burk和Anacardium giganteum种子的提取物。为了搜索植物提取物的协同作用以增强抗生素对MRSA的作用，Matayba秀丽隐杆线虫叶/茎的提取物，Campsiandra Commosa Burk和Cupania Americana Aeriial Parter均表现出显着的活性。提取物的生物测定引导分离使我们能够表征某些活性成分。在这些药用植物提取物中，我们已经分离并确定了15种新物质的结构。