CLONING OF AVIAN CLOCK GENES AND ANALYSIS OF CIRCADIAN CLOCK AND PHOTOTERIODIC TIME MEASUREMENT

鸟类时钟基因的克隆及昼夜节律分析和光敏时间测量

基本信息

批准号：
12460123
负责人：
EBIHARA Shizufumi
金额：
$ 9.09万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460123/
关键词：
circadian rhythm Photoperiodic response Clock gene Japanese quail Melatonin Ovary Pineal SCN 光周性

项目摘要

Circadian rhythms, observed in a wide variety of physiological and behavioral parameters, are important for adaptation to natural environment in organisms living on the earth. Recently we have succeeded in cloning avian clock genes (qPer2, qPer3, qClock) which constitute the core of the circadian clock in Japanese quail. In this project, we have analyzed avian circadian clock and photoperiodic time measurement with these clock genes. The results are summarized as follows. (1) Analysis of clock gene expression : Clock genes are expressed in the pineal gland, the retina and many peripheral tissues. In particular, qPer2 and qPer3 show distinct circadian rhythms (Mol Brain Res, 2000). (2) Identification of the avian SCN : The avian SCN, the site of which had not been determined for more than 20 years, was identified with the expression of clock genes (Am J Physiol, 2001). (3) Effects of melatonin on the expression of clock gene in the avian SCN : Because melatonin is involved in the regula … More tion of avian circadian rhythms, effects of melatonin administration on the clock gene expression in the SCN were examined. The results indicate that melatonin does not affect the transcription of clock genes, but rather melatonin seems to affect the coupling among the SCN cells (Europ J Neurosci, 2002), (4) Clock genes and photoperiodic response : To examine how clock genes are involved in photoperiodic time measurement, the expression patterns of clock genes in the pineal, the SCN and the infundibular nucleus of quail under long and short days were examined. The results indicate that the patterns alter in the SCN and the pineal, but not in the infundibular nucleus which is the center for the photoperiodic response. (5) The function of clock genes in the ovary : Clock gene expression was examined in the ovary, and the results suggest that the clock in the ovary regulates the ovulation. (6) Ontogeny of clock genes : Using chicken embryo, ontogeny of clock gene expression was studied. The results indicate that light-dark cycles are required for the expression of clock gene because under constant darkness no expression was observed. (7) Novel gene controlling photoperiodic response : Weisolated a novel gene regulating photoperiodic response in Japanese quail. Less

在各种物理和行为参数中观察到的昼夜节律对于适应生活在地球上的生物体的自然环境非常重要。最近，我们成功地克隆了鸟钟基因（QPER2，QPER3，QCLOCK），它构成了日本鹌鹑的昼夜节律核心。在这个项目中，我们通过这些时钟基因分析了鸟类昼夜节律的时钟和光周期时间测量。结果总结（1）时钟基因表达的分析：时钟基因在松果体，视网膜和许多周围组织中表达。特别是，QPER2和QPER3显示出不同的昼夜节律（Mol Brain Res，2000）。（2）鉴定鸟类SCN：鸟类SCN（其位点已有20年以上，其位点已经被确定了，它被鉴定出了时钟基因的表达（Am J Physiol，2001）。（3）褪黑激素对鸟类SCN中时钟基因表达的影响：因为褪黑激素参与了探测……鸟类昼夜节律的更多影响，因此检查了褪黑激素给药对SCN中钟基因表达的影响。 The results indicate that melatonin does not affect the transcription of clock genes, but rather melatonin seems to affect the coupling among the SCN cells (Europ J Neurosci, 2002), (4) Clock genes and photoperiodic response: To examining how clock genes are involved in photoperiodic time measurement, the expression patterns of clock genes in the pineal, the SCN and the infundibular nuclearus of quail under long and检查了短时间。结果表明，模式在SCN和松果体中改变，但在漏斗性核us中没有改变，这是光周期反应的中心。（5）在卵巢中检查了卵巢基因：时钟基因表达中时钟基因的功能，结果表明卵巢中的时钟调节排卵。（6）时钟基因的个体发育：使用鸡胚胎，时钟基因表达的个体发育。结果表明，在恒定黑暗下未观察到表达时钟基因的表达需要浅黑暗循环。（7）控制光周期反应的新基因：在日本鹌鹑中引起了新的基因调节光周期反应。较少的