CLONING OF AVIAN CLOCK GENES AND ANALYSIS OF CIRCADIAN CLOCK AND PHOTOTERIODIC TIME MEASUREMENT

鸟类时钟基因的克隆及昼夜节律分析和光敏时间测量

基本信息

批准号：
12460123
负责人：
EBIHARA Shizufumi
金额：
$ 9.09万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460123/
关键词：
circadian rhythm Photoperiodic response Clock gene Japanese quail Melatonin Ovary Pineal SCN 光周性

项目摘要

Circadian rhythms, observed in a wide variety of physiological and behavioral parameters, are important for adaptation to natural environment in organisms living on the earth. Recently we have succeeded in cloning avian clock genes (qPer2, qPer3, qClock) which constitute the core of the circadian clock in Japanese quail. In this project, we have analyzed avian circadian clock and photoperiodic time measurement with these clock genes. The results are summarized as follows. (1) Analysis of clock gene expression : Clock genes are expressed in the pineal gland, the retina and many peripheral tissues. In particular, qPer2 and qPer3 show distinct circadian rhythms (Mol Brain Res, 2000). (2) Identification of the avian SCN : The avian SCN, the site of which had not been determined for more than 20 years, was identified with the expression of clock genes (Am J Physiol, 2001). (3) Effects of melatonin on the expression of clock gene in the avian SCN : Because melatonin is involved in the regula … More tion of avian circadian rhythms, effects of melatonin administration on the clock gene expression in the SCN were examined. The results indicate that melatonin does not affect the transcription of clock genes, but rather melatonin seems to affect the coupling among the SCN cells (Europ J Neurosci, 2002), (4) Clock genes and photoperiodic response : To examine how clock genes are involved in photoperiodic time measurement, the expression patterns of clock genes in the pineal, the SCN and the infundibular nucleus of quail under long and short days were examined. The results indicate that the patterns alter in the SCN and the pineal, but not in the infundibular nucleus which is the center for the photoperiodic response. (5) The function of clock genes in the ovary : Clock gene expression was examined in the ovary, and the results suggest that the clock in the ovary regulates the ovulation. (6) Ontogeny of clock genes : Using chicken embryo, ontogeny of clock gene expression was studied. The results indicate that light-dark cycles are required for the expression of clock gene because under constant darkness no expression was observed. (7) Novel gene controlling photoperiodic response : Weisolated a novel gene regulating photoperiodic response in Japanese quail. Less

在各种生理和行为参数中观察到的昼夜节律对于生活在地球上的生物体适应自然环境非常重要，最近我们成功克隆了构成生物钟核心的鸟类时钟基因（qPer2、qPer3、qClock）。在本项目中，我们利用这些时钟基因分析了鸟类的生物钟和光周期时间测量，结果总结如下。时钟基因在松果体、视网膜和许多外周组织中表达，特别是 qPer2 和 qPer3 显示出不同的昼夜节律（Mol Brain Res，2000）（2）鸟类 SCN 的识别：鸟类 SCN 的位点。 20多年来一直没有被确定，但与时钟基因的表达有关（Am J Physiol，2001）（3）的影响。褪黑激素对鸟类 SCN 时钟基因表达的影响：由于褪黑激素参与鸟类昼夜节律的调节，因此检查了褪黑激素给药对 SCN 时钟基因表达的影响，结果表明褪黑激素不会影响。时钟基因的转录，而褪黑激素似乎影响 SCN 细胞之间的耦合（Europ J Neurosci，2002），（4）时钟基因和光周期响应：为了研究时钟基因如何参与光周期时间测量，研究了鹌鹑在长日照和短日照下的松果体、视交叉上核和漏斗核中时钟基因的表达模式，结果表明这些模式在光照下发生变化。 SCN 和松果体，但不在漏斗核中，漏斗核是光周期反应的中心。 (5) 卵巢中时钟基因的功能：检查了卵巢中的时钟基因表达。结果表明，卵巢中的生物钟调节排卵。 (6)生物钟基因的个体发育：利用鸡胚，研究了生物钟基因表达的个体发育。结果表明，生物钟基因的表达需要光暗周期。 (7)控制光周期反应的新基因：我们在日本鹌鹑中分离到一个控制光周期反应的新基因。