Evaluation of Internal Structure of the Articular Cartilage in Terms of ^1H-NMR Relaxation Behavior

根据 ^1H-NMR 松弛行为评估关节软骨的内部结构

基本信息

批准号：
14370470
负责人：
TOKUNAGA Daisaku
金额：
$ 7.17万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370470/
关键词：
Articular cartilage Collagen fiber MRI T1 relaxation time T2 relaxation time DQF NMR Anisotropy of collagen fiber Orientation of collagen fiber

项目摘要

The structural characteristics of articular cartilage were analyzed usnig ^1H-longitudinal (T_1) and transverse (T_2) relaxation times as measured by fast-inversion-recovery and multi-spin-echo magnetic resonance imaging(MRI). Pairs of cartilage-bone plugs from weight bearing and non-weight bearing regions were dissected from 15 medial femoral condyles and were subjected to NMR measurements with and without static loads (0.15-1.0 MPa). The T_1 of the cartilage with no load showed a maximum value just beneath the articular surface and this value decreased gradually towards the deeper zones. The T_2 of the same cartilage showed a maximum value at or just beneath the articular surface which decreased rapidly towards the intermediate zone yet increased again in the deepest zone. The increase of T_2 in the deepest zone was more greatly pronounced in the weight bearing region than in the non-weight bearing region. These layer-dependent differences in the T_1 and T_2 could account for the laminar appearance of the articular cartilage in the MR images. Under static loads, the decrease of T_1 in the transitional zone (from just beneath the articular surface to the intermidiate zone) was significant. Because the T_1 has a positive correlation with the water content, this decrease of T_1 might signify that the largest water loss occurs in the transitional zone. These results suggest that the transitional zone might be attenuating the mechanical stress in the joint, and the expressed water from the cartilage could substantially contribute to the lubrication of the joint.

使用通过快速反转恢复和多自旋回波磁共振成像（MRI）测量的^1H纵向（T_1）和横向（T_2）弛豫时间来分析关节软骨的结构特征。从 15 个内侧股骨髁上切下来自承重和非承重区域的成对软骨骨塞，并在有和没有静载荷 (0.15-1.0 MPa) 的情况下进行 NMR 测量。空载软骨的T_1在关节面正下方显示出最大值，并且该值向更深的区域逐渐减小。同一软骨的T_2在关节面处或关节面正下方显示出最大值，该最大值朝中间区迅速下降，但在最深区再次增加。最深区T_2的增加在负重区域比非负重区域更加明显。 T_1 和 T_2 中的这些层相关差异可以解释 MR 图像中关节软骨的层状外观。在静载荷作用下，过渡区（从关节面正下方到中间区）的T_1下降显着。由于T_1与含水量呈正相关，因此T_1的下降可能意味着最大的失水发生在过渡带。这些结果表明，过渡区可能会减弱关节中的机械应力，并且软骨中挤出的水可以极大地有助于关节的润滑。