MRI evaluation of cartilage protein content with multi-component T1rho/T2 at 7T

7T 多成分 T1rho/T2 软骨蛋白含量的 MRI 评估

基本信息

批准号：
8398141
负责人：
Cory R. Wyatt
金额：
$ 5.22万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-01 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8398141
关键词：
Acceleration Adult Age American Area Binding Biochemical Bovine Cartilage Calcified Cartilage Cattle Chemicals Clinical Collagen Collagen Fiber Creatine Data Data Quality Data Set Degenerative polyarthritis Detection Development Early Diagnosis Enzymes Feasibility Studies Future Generations Glutamates Human Image Image Analysis Individual Knee Knee Osteoarthritis Learning Life Expectancy Magnetic Resonance Imaging Measurement Measures Medical Meniscus structure of joint Morphologic artifacts Motion Noise Obesity Occupations Patients Prevention Proteins Proteoglycan Public Health Radial Relaxation Reporting Research Resolution Retirement Sampling Scanning Sepharose Signal Transduction Site Spatial Distribution Staging Structural Protein Structure Testing Time Tissues Validation Water Work absorption articular cartilage bone cost design disability human tissue improved in vivo knee replacement arthroplasty novel novel diagnostics novel marker research study tool volunteer

项目摘要

DESCRIPTION (provided by applicant): We propose to develop a novel UTE-T1rho magnetic resonance imaging (MRI) sequence at 7 Tesla that will provide multiexponential measures of T1rho and T2 in the superficial, deep, and calcified layers of cartilage. T1rho relaxation values have been correlated with proteoglycan concentration in cartilage, while T2 relaxation has been shown to relate to the collagen fiber structure. The use of 7 Tesla imaging will allow for higher signal to noise ratio and possibly higher resolution, potentially improving the accuracy of T1rho measurement. Additionally, T1rho sensitivity will increase at 7 Tesla, potentially allowing for detection of smaller changes. Aim 1 will focus on development of the combined UTE-T1rho sequence at 3 Tesla and 7 Tesla. Initial work will focus on conversion of individual UTE and T1rho sequences from 3 Tesla to 7 Tesla. The sequences will then be combined at 3 Tesla to learn lessons on a less challenging application. Lastly, the lessons learned at 3 Tesla will be used to combine the sequences at 7 Tesla. In Aim 2, the sequences developed in Aim 1 will be validated in experiments with phantoms, bovine cartilage, and human cartilage. Initial experiments will be performed in phantoms with varying concentrations of agarose to validate the T1rho and UTE measures of the sequences. Experiments in excised cartilage samples from 6-18 month bovine knees will be imaged with the combined sequence and the multiexponential T1rho/T2 values will be examined. Enzyme treatments will be performed to help separate the contributions from collagen and proteoglycans. Lastly, human cartilage samples from patients undergoing total knee replacement will be imaged with the combined sequence to determine multiexponential T1rho/T2 values in human tissue. In Aim 3, a feasibility study of twenty volunteers will be conducted to test and optimize the combined sequences in vivo and provide preliminary data for future studies. The volunteers will be split evenly between four groups. Group 1 will consist of healthy controls between the age of 20-35, Group 2 will consist of volunteers (age 35-60) with a Kellgren-Lawrence (KL) score of 0, Group 3 will consist of volunteers with KL=1-2 (mild OA), and Group 4 will consist of volunteers with KL=3-4 (severe OA). Volunteers in Group 1 will be imaged with the combined sequence at 3 Tesla and 7 Tesla, while the other groups will be imaged only at 7 Tesla. Multiexponential T1rho/T2 values will be examined for all volunteers. Relevance Currently, T1rho imaging at 3 Tesla is used as a marker for proteoglycan loss in cartilage during the early stages of OA. This work will improve the sensitivity of these measurements and allow for T1rho measurements in areas of cartilage with short T2, such as the deep and calcified layers. Additionally, the sequences developed will allow for multiexponential fitting of the T1rho and T2 values, potentially providing new markers for cartilage degeneration. All of these improvements will also potentially allow for improved detection of biochemical changes to cartilage due to the onset of OA. PUBLIC HEALTH RELEVANCE: This work will improve the sensitivity of T1rho measurements of knee cartilage and allow for these measurements in areas of cartilage with short T2, such as the deep and calcified layers. Additionally, the sequences developed will allow for multiexponential fitting of the T1rho and T2 values, potentially providing new markers for cartilage degeneration. All of these improvements will also potentially allow for improved detection of biochemical changes to cartilage due to the onset of OA.

描述（由申请人提供）：我们提议在7 Tesla处开发一种新型的UTE-T1RHO磁共振成像（MRI）序列，该序列将在表面，深层和钙化的软骨层中提供T1rho和T2的多级测量。 T1RHO弛豫值已与软骨中的蛋白聚糖浓度相关，而T2弛豫已显示与胶原纤维结构有关。使用7种特斯拉成像将允许更高的信号与噪声比，并可能提高分辨率，从而有可能提高T1RHO测量的准确性。另外，T1RHO敏感性将在7 Tesla时增加，有可能允许检测较小的变化。 AIM 1将重点放在3特斯拉和7特斯拉的联合UTE-T1RHO序列的开发上。最初的工作将集中于将单个UTE和T1RHO序列从3个特斯拉转变为7特斯拉。然后，将在3个特斯拉组合这些序列，以在不具有挑战性的应用程序上学习课程。最后，在3个特斯拉的经验教训将用于将序列组合在7特斯拉处。在AIM 2中，在AIM 1中开发的序列将在幻象，牛软骨和人软骨的实验中得到验证。最初的实验将在具有变化浓度的琼脂糖的幻像中进行，以验证序列的T1RHO和UTE度量。将使用组合序列成像6-18个月牛膝膝膝膝膝膝膝膝的软骨样品中的实验，将检查多Exparential T1RHO/T2值。将进行酶处理，以帮助将贡献与胶原蛋白和蛋白聚糖分开。最后，将与联合序列成像的患者的人体软骨样品将成像，以确定人体组织中的多型T1RHO/T2值。在AIM 3中，将对二十名志愿者进行可行性研究，以测试和优化体内组合序列，并为未来的研究提供初步数据。志愿者将在四组之间平均分配。第1组将由20-35岁之间的健康对照组成，第2组将由志愿者（35-60岁）组成，Kellgren-Lawence（KL）得分为0，第3组将由KL = 1-的志愿者组成。 2（轻度OA）和第4组将由KL = 3-4（严重OA）的志愿者组成。第1组中的志愿者将以3特斯拉和7特斯拉的组合序列成像，而其他组仅在7特斯拉处成像。将检查所有志愿者的多符号T1RHO/T2值。目前，在OA的早期阶段，将3特斯拉的T1RHO成像用作软骨中蛋白聚糖损失的标记。这项工作将提高这些测量值的敏感性，并允许在短T2（例如深层和钙化层）软骨区域进行T1RHO测量。此外，开发的序列将允许T1RHO和T2值的多符合拟合，从而有可能为软骨变性提供新的标记。所有这些改进还将有可能改善由于OA发作而导致软骨的生化变化的检测。公共卫生相关性：这项工作将提高膝盖软骨测量值的敏感性，并允许在短T2（例如深层和钙化层）软骨区域进行这些测量。此外，开发的序列将允许T1RHO和T2值的多拟合拟合，从而有可能为软骨变性提供新的标记。所有这些改进还将有可能改善由于OA发作而导致软骨的生化变化的检测。