Single-Microparticle Based Analyses under Solution Flow Conditions

溶液流动条件下基于单微粒的分析

基本信息

批准号：
11554032
负责人：
KIM Haeng-boo
金额：
$ 7.94万
依托单位：
The University of Tokyo (2000-2001)Hokkaido University (1999)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11554032/
关键词：
microspectroscopy laser-trapping flow cell extraction solid phase concentration microparticle dye staining of living cell イオン交換樹脂

项目摘要

Microspectroscopy and microelectrochemical systems combined with a fluid manifold were developed to manipulate : and analyze single microparticles such as polymer beads, oil droplets, living cells, and so forth. By using a flow cell set on a microscope stage, an aqueous solution containing microparticles is introduced to the cell. With a single particle being fixed by laser-trapping for polymer beads, on the top end of a microcapillary for oil-droplets, or on the wall by adhesion for living-cells, other non-fixed particles are pumped out completely by flow of sufficient amount of water to the cell. These procedures can hold exclusively the single particle in the cell. By using an appropriate fluid manifold, furthermore, an arbitrary reagent can be introduced to the cell, so that a temporal profile of chemical/physical responses of the particle would be monitored by microspectroscopic and/or microelectrochemical method(s) without interference by other particles. Three different chemical/biochemical processes were studied under solution-flow conditions :1) Ion-exchange dynamics of a single ion-exchange-resin bead by laser trapping-absorption microspectroscopy.2) Liquid/liquid extraction dynamics of a single oil-droplet by microinjection - microspectroscopy.3) Dye-staining dynamics of a single living-cell (Saccharomyces cerevisiae)The ion-exchange, the extraction, or the staining rate and efficiency were discussed in terms of the solution flow rate and the particle diameter.

开发了微光谱和微电化学系统与流体歧管的结合来操纵：分析单个微粒，例如聚合物珠，油滴，活细胞等。通过在显微镜阶段使用流动池集，将含有微粒的水溶液引入细胞。通过通过激光捕获聚合物珠的单个粒子，在微毛细管的顶端，油滴管的顶端或在壁上通过粘附在壁上的活细胞，其他非固定颗粒被足够量的水流向细胞而完全泵出。这些过程可以完全保存单元中的单个粒子。此外，通过使用适当的液体歧管，可以将任意试剂引入细胞，以便通过微光谱和/或微电化学方法监测颗粒的化学/物理反应的时间谱，而不会受到其他颗粒的干扰。在溶液流条件下研究了三种不同的化学/生化过程：1）通过激光吸收 - 吸收微光谱的激光诱捕 - 液体/液体萃取动力学，单个油的液体 - 脱落动力学通过微量注射 - 摄影 - 微光谱 - 微谱 - 单个染色器（3）染色（3）染色（3）染色（3）用溶液流速和颗粒直径讨论了离子 - 交换，提取率和效率的离子交换。