Nanoseond Ultra-Rapid Freeze Quenching for Time-resolved Structural Biology

用于时间分辨结构生物学的纳秒超快速冷冻淬火

• 批准号: 451906961
• 负责人: Professor Dr. Harald Schwalbe
• 金额: --
• 依托单位: Institut für Organische Chemie und Chemische Biologie
• 依托单位国家: 德国
• 项目类别: New Instrumentation for Research
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/451906961?language=en
• 关键词: Nanoseond; Ultra; Rapid; Freeze; Quenching

The application aims to develop instrumentation for the time-resolved studies of biochemical processes, yielding 3D molecular movies recorded with atomic resolution in real time. Application targets regular and custom-made equipment for pre-steady-state triggering, sampling, probing and detection, including optical, cryo-sedimentation and ultra-high vacuum systems, UHPLC sample delivery and the computation cluster upgrade. Such instrumentation will be essential for time-resolved structural biology, in particular solid state NMR and cryo-EM.

该应用程序旨在为生化过程的时间分辨研究开发仪器，并实时产生以原子分辨率记录的3D分子电影。应用程序针对常规和定制设备，用于稳态触发，采样，探测和检测，包括光学，冷冻测量和超高真空系统，UHPLC样品输送和计算集群升级。这种仪器对于时间分辨的结构生物学，尤其是固态NMR和Cryo-EM至关重要。