NMR and biophysical characterization of structure and dynamics of µ-proteins and their complexes.

μ-蛋白质及其复合物的结构和动力学的核磁共振和生物物理表征。

• 批准号: 379644268
• 负责人: Professor Dr. Harald Schwalbe
• 金额: --
• 依托单位: Institut für Organische Chemie und Chemische Biologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/379644268?language=en
• 关键词: NMR; biophysical; characterization; structure; dynamics; NMR; biophysical; characterization; structure; dynamics

Small proteins are encoded by small open reading frames (sORFs) and consist of around 60 amino acids. Diverse cellular functions have been linked to small proteins including cell division, transport and kinase regulation, morphogenesis and stress response. In our project, we will investigate the structure and dynamics of small proteins and their complexes by nuclear magnetic resonance (NMR) spectroscopy. We will continue our fruitful collaborations within SPP2002 with more than ten different groups. Beside characterizing the invidual small proteins using a workflow successfully established in the first funding period, we will also put focus on investigating complexes of small protein with (larger) proteins, DNA, RNA or lipids.

小蛋白质由小的开放式阅读框（SORF）编码，由大约60个氨基酸组成。各种细胞功能与小蛋白质有关，包括细胞分裂，运输和激酶调节，形态发生和应激反应。在我们的项目中，我们将通过核磁共振（NMR）光谱研究小蛋白质及其复合物的结构和动力学。我们将继续在SPP2002中与十多个不同的群体继续我们的富有成果的合作。除了使用第一个资金期间成功建立的工作流程表征不景气的小蛋白外，我们还将重点放在研究与（较大）蛋白质，DNA，RNA或脂质的小蛋白质的复合物。