Development of bio electron microscopy under ultra low electron dose conditions

超低电子剂量条件下生物电子显微镜的发展

基本信息

批准号：
18206007
负责人：
TAKAI Yoshizo
金额：
$ 17.06万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18206007/
关键词：
tansmission electron microscope aberration correction phase reconstruction 3 dimensional Fourier filtering radiation damage low electron dose DNA

项目摘要

Biological samples that are composed of light atoms show very little contrast in transmission electron microscopy (TEM) and only a small electron dose is tolerable for taking the image because the structures are easily damaged by electron beam irradiation. Furthermore, an in-focus image shows little contrast due to a lack of an ideal phase plate in conventional TEM. Therefore, high-resolution observation of biological samples is extremely difficult, although the direct observation of unstained molecular structures seems to be one of the promising approaches for identifying the localized structures of individual molecules and for understanding their functions. The three-dimensional Fourier filtering method (3DFFM) developed by Ikuta and Kawasaki seems to be the most promising approach for this purpose. In the method, a 3D Fourier transform is performed for a number of recorded through-focus images and the obtained 3D Fourier spectrum is multiplied by a filtering shape function with aberration correction factors. Finally, the phase image is obtained by performing an inverse 3D Fourier transform on the corrected 3D Fourier spectrum.In the preset research project, the molecular structure of deoxyribonucleic acid (DNA) fibers were observed by a phase reconstruction method called three-dimensional Fourier filtering using a transmission electron microscope. The characteristic helical structure and the spacing of adjacent base pairs of DNA were partially resolved due to an improved S/N ratio and resolution enhancement by the phase reconstruction although the molecular structure was damaged by the electron beam irradiation. In the spherical aberration-free phase images, the arrangements of single atom-sized spots forming sinusoidal curves were sometimes observed, which seem to be the contrast originating in the sulfur atoms along the main chains

由光原子组成的生物样品在透射电子显微镜（TEM）中几乎没有对比度，并且仅一个小电子剂量可以忍受拍摄图像，因为这些结构很容易被电子束照射损坏。此外，由于常规TEM中缺乏理想的相板，对比度的对比度很小。因此，尽管直接观察未染色的分子结构似乎是识别单个分子的局部结构并了解其功能的有前途的方法之一，但对生物样品的高分辨率观察非常困难。 Ikuta和川崎开发的三维傅立叶过滤方法（3DFFM）似乎是为此目的最有前途的方法。在该方法中，对许多记录的透射型图像进行了3D傅立叶变换，并将获得的3D傅立叶光谱乘以具有异差校正因子的过滤形状函数。最后，通过在校正后的3D傅立叶光谱上进行反相反的3D傅立叶变换来获得相位图像。在预设研究项目中，通过使用传输电子显微镜使用三维傅立叶滤波器，通过一种称为三维傅立叶滤波的相位重建方法观察到脱氧核糖核酸（DNA）纤维的分子结构。由于电子束照射受到了分子结构的损害，但由于S/N比的提高和分辨率增强，DNA的特征性螺旋结构和相邻碱基对的间距部分被部分解析。在无球差相图像中，有时会观察到形成正弦曲线的单个原子大小斑点的排列，这似乎是沿主要链的硫原子中的对比

项目成果

期刊论文数量（59）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Development of Micro-aperture for Annular Pupil on Electron Optics by Focused Ion Beam Technique(in Japanese)

利用聚焦离子束技术开发电子光学环形光瞳微孔径（日文）

DOI：
发表时间：
2007
期刊：
J. Vac. Soc. Jpn. 50
影响因子：
0
作者：
M. Fujiwara;et. al.
通讯作者：
et. al.

Interpretation of Cs-corrector System with Twin Hexapoles and Transfer Doublet based on Geometrical Optics Theory

基于几何光学理论的双六极杆和传递双合态铯校正系统的解释

DOI：
发表时间：
2007
期刊：
Proc. of International Symposium on Eco Topia Science 2007 1
影响因子：
0
作者：
T. Kawasaki;M. Ichihashi;T. Matsutani;Y. Kimura;and T. Ikuta
通讯作者：
and T. Ikuta

「研究成果報告書概要(和文)」より

摘自《研究结果报告摘要（日文）》

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野幹雄;星野幹雄
通讯作者：
星野幹雄

Development of Annular Pupil for Scanning Transmission Electron Microscope by Focused Ion Beam Technique

聚焦离子束技术扫描透射电子显微镜环形光瞳的研制

DOI：
发表时间：
2007
期刊：
Advances in Applied Plasma Science Vol.6
影响因子：
0
作者：
Takaomi Matsutani;Masaki Taya;Takashi Ikuta;Tetsuya Fujimura;Hrihiko Inui;Takeo Tanaka;Yoshihide Kimura;Yoshizo Takai;Tadahiro Kawasaki;Mikio Ichihashi
通讯作者：
Mikio Ichihashi