Applying ferrite nanobeads to medical diagnosis, therapy and analysis

将铁氧体纳米珠应用于医学诊断、治疗和分析

基本信息

批准号：
15209019
负责人：
ABE Masanori
金额：
$ 32.45万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15209019/
关键词：
Magnetic beads Bio-screening Bio-sensing MRI contrast agent Hyperthermia Anticancer drug DNA Ferrite nanobeads 磁性ビーズ DNA検出 MRI造影剤フェライト粒子 GMA 抗体 MMI造影フェライトナノビーズ IgG抗体 CEA抗体

项目摘要

1. Development of size controlled ferrite nanobeadsIron-ferrite nanoparticles with controlled size of 10-100 nm were successfully synthesized by our newly developed methods called co-precipitation, partial-oxidation, and seed-growth methods.2. Development of functional-polymer coated ferrite nanobeadsThe iron-ferrite nanoparticles were successfully coated with poly-GMA, which exhibits minimal non-specific adsorption of proteins, by a co-polymerization method and a novel, soap-free polymerization method.3. Development of polymer coated iron nanobeadsMetallic iron nanoparticles (7-20 nm in size), which have magnetization two times stronger than that of ferrite particles and are advantageous for magnetic separation in bio-screening, were successfully coated with poly-GMA.4. Application to analysis by bio-screening systemThe poly-GMA coated ferrite nanobeads were conjugated with physiologically active substances such as anticancer antibodies and recombinant proteins containing tagged Prote … More in-G. Utilizing the beads, we successfully captured physiologically active substances that are complementary to those conjugated onto the beads. This revealed our beads are fitted for magnetic carriers for high throughput bio-screening.5. Application to diagnosis by Hall bio-sensingpoly-GMA coated ferrite nanobeads were conjugated with single strand DNA, which were immobilized onto the surface of a Hall sensor via hybridization with the complementary DNA single strand fixed on the sensor surface. This revealed our beads are applicable to magnetic carriers for DNA analysis.6. Application to diagnosis by contrast enhanced MRIWe successfully modified the surfaces of the ferrite nanobeads with a variety of functional molecules, and discovered candidate materials for high performance MRI contrast agent.7. Application to anticancer magnetic hyperthermiaUtilizing the ferrite nanobeads as heat source by magnetic-field induction at an non-invasive frequency of 120 kHz, we successfully killed human colon cancer cells. We thus revealed that our beads are applicable to magnetic carriers for non-invasive, anticancer magnetic hyperthermia. Less

1. 尺寸可控铁氧体纳米珠的开发通过我们新开发的共沉淀法、部分氧化法和种子生长法成功合成了尺寸可控的10-100 nm铁氧体纳米粒子。2．铁氧体纳米珠铁氧体纳米颗粒成功地涂有聚 GMA，其对蛋白质的非特异性吸附极小。共聚方法和新型无皂聚合方法。 3. 开发聚合物包覆的铁纳米珠（尺寸为7-20 nm），其磁化强度是铁氧体颗粒的两倍，有利于磁分离。在生物筛选中，成功地包覆了聚GMA。4．在生物筛选系统分析中的应用聚GMA包覆的铁氧体纳米珠与生理活性物质缀合。利用珠子，我们成功捕获了与珠子上缀合的生理活性物质，例如抗癌抗体和重组蛋白，这表明我们的珠子适合用于高通量生物的磁性载体。 -筛选.5.霍尔生物传感诊断中的应用将聚GMA包被的铁氧体纳米珠与单链DNA结合，通过固定化到霍尔传感器的表面与固定在传感器表面的互补DNA单链杂交，这表明我们的磁珠适用于DNA分析的磁性载体。6.我们成功地用多种功能分子修饰了铁氧体纳米磁珠的表面。并发现了高性能MRI造影剂的候选材料。7．在抗癌磁热疗中的应用利用铁氧体纳米珠作为热源，通过120kHz的非侵入性磁场感应，我们发现了磁性热疗的应用。成功杀死了人类结肠癌细胞，从而表明我们的磁珠适用于磁性载体，用于非侵入性抗癌磁热疗。

项目成果

期刊论文数量（249）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A specific genetic alteration on chromosome 6 in ulcerative colitis-associated colorectal cancers.

DOI：
发表时间：
2003-07
期刊：
Cancer research
影响因子：
11.2
作者：
T. Ezaki;Mamoru Watanabe;N. Inoue;T. Kanai;H. Ogata;Y. Iwao;H. Ishii;T. Hibi
通讯作者：
T. Ezaki;Mamoru Watanabe;N. Inoue;T. Kanai;H. Ogata;Y. Iwao;H. Ishii;T. Hibi

磁性ビーズのバイオ・環境技術への応用展開

磁珠在生物/环境技术中的应用

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Takahashi;A.;et. al.;B.Jeyadevan
通讯作者：
B.Jeyadevan

The VP2/VP3 minor capsid plotein of SV40 promotes the in vitroassembly of the major capsid protein VPl into particles.

SV40的VP2/VP3次要衣壳蛋白促进主要衣壳蛋白VP1在体外组装成颗粒。

DOI：
发表时间：
期刊：
J.Biol.Chem. in press
影响因子：
0
作者：
Kawano;M.;Inoue;T.;Tsukamoto;H.;Takaya;T.;Enomoto;T.;Takahashi;R.U.;Yokoyama;N.;Yamamoto;N.;Nakanishi;A.;Imai;T.;Wada;T.;Kataoka;K.;Handa;H.
通讯作者：
H.

H.Nohara: "Association between the interleukin-1 receptor antagonist polymorphism and ulcerative colitis with younger age at diagnosis"Immunol Lett. 90・1. 53-57 (2003)

H.野原：“白细胞介素1受体拮抗剂多态性与诊断时年龄较小的溃疡性结肠炎之间的关联”Immunol Lett 90・1（2003）。