Magnetic probe with ferrite couples to bioligands for diagnostic and clinical uses

带有铁氧体的磁性探针与生物配体偶联，用于诊断和临床用途

基本信息

批准号：
12309004
负责人：
ABE Masanori
金额：
$ 20.54万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12309004/
关键词：
medical magnetic beads ferrite beads IGG antibody anticancer drug hyperthermia magnetic bioprobe antibody antigen 蛍光磁性プローブマグネタイトバイオ・リガンドバイオ高分子スピネル型 BSA CMC 生体高分子磁気分離

项目摘要

1. Development of novel medical ferrite Nano-BeadsWe established the technique to immobilize physiologically active molecules onto the surfaces of ferrite (Fe_3O_<4+8>) nano beads (10-15 run in diameter) during the beads are synthesized from an aqueous solution. We prepared medical magnetic nano beads on the surfaces of which we strongly immobilized such physiologically active molecules as Trypsin FITC-avidin, anti-cancer drugs, glycidyl methacrylate (GMA), amino acids (e.g. aspartic acid) and similar-strusture molecules, polyacryl amino acid, etc.2. We revealed that in the above-mentioned fenite nano beads the physiologically active molecules are strongly couples to ferrite intermediated by S-H and COOH groups of the amino acids or similar-structure molecules contained in the physiologically active molecules. We named the intermediating amino acids (and similar structured molecules) connectors. Also we called the medical nano beads (with physiologically active molecules coupled to the … More surfaces intermediated by the connectors) "Connectors-Bonded Magnetic (CBM)" nano beads. Furthermore, we developed CBM nano beads having surfaces coated with (1)connectors and (2)polymers exhibiting low sensitivity to non-specific absorption of physiologically active molecules ; on their surfaces we can easily immobilize physiologically active molecules by biochemical technique.3. Preparation of human-type antibody and novel ferrite bioprobesWe have prepared human-type IGG antibody to human colon cancer cells by replacing the constant region of mouse monoclonal IGG antibody with that of human IGG antibody. The human-type IGG antibody fixed on the ferrite beads selectively reacted with the antigens present on human colon cancer cell membrane, which were visualized by staining with anti-IGG antibody conjugated with fluorescence. This shows that die IGG antibody-fixed ferrite beads are utilized for detection of cancer as diagnostic magnetic bioprobes.4. Power absorption rate of ferrite nano beads for hyperthermiaWe found that the fenite nano beads prepared in this study have specific power absorption rate for AC magnetic field induction 1.4 times higher than that of conventional ferrite beads. This indicates that our ferrite nano beads are advantageous for applying to hyperthermia. Less

1。开发新型的铁氧体纳米 - 贝德斯威，建立了固定活跃的活性分子到铁氧体（Fe_3o_ <4+8>）纳米珠（直径为10-15）的铁素体表面（直径为10-15）。准备的磁性纳米珠在抗癌药物，抗癌药物，甲基丙烯酸糖基（GMA），氨基酸（例如天冬氨酸）和相似感性分子，多丙烯酸氨基酸等中的磁性纳米珠在上述矿石纳米珠中，生理的活性分子是由氨基酸的S-H和COOH组的强烈夫妻。连接器。在其表面上有氧化的活性分子； 3人类IgG抗体。在本研究中制备的Fenite纳米珠具有针对动力场的规格吸收，以提高传统的铁氧体珠的1.4倍