400 MHz NMR console für solid-state-NMR

用于固态 NMR 的 400 MHz NMR 控制台

• 批准号: 447235569
• 金额: --
• 依托单位: Goethe-Universität Frankfurt am Main
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/447235569?language=en
• 关键词: 400; MHz; NMR; console; solid

DNP-enhanced solid-state NMR offers an increase in sensitivity by orders of magnitude. It opens the door to new, unconventional NMR experiments and especially the analysis of samples with low spin numbers. We use this technique especially for the study of membrane proteins and in particular for probing subunit interactions within large complexes, for analyzing low-populated intermediate states and for small molecules in complex with transporters or receptors. Protein families of interest are GPCRs, ABC transporter, kinases and photoreceptors. In order to continue and to expand these studies, an outdated NMR console within the excising DNP-NMR spectrometer needs to be replaced, which is the aim of this proposal.

DNP增强的固态NMR通过数量级提高了灵敏度。它为新的，非常规的NMR实验打开了大门，尤其是对较低自旋数的样品的分析。我们特别用于研究膜蛋白的研究，尤其是用于探测大型复合物中的亚基相互作用，用于分析矮个含量的中间状态和与转运蛋白或受体复合物中的小分子。感兴趣的蛋白质家族是GPCR，ABC转运蛋白，激酶和感光体。为了继续并扩大这些研究，需要更换脱离DNP-NMR光谱仪内过时的NMR控制台，这是该提案的目的。