Superconducting NMR Magnet 400 MHz

超导核磁共振磁体 400 MHz

• 批准号: 528284074
• 金额: --
• 依托单位: Martin-Luther-Universität Halle-Wittenberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/528284074?language=en
• 关键词: Superconducting; NMR; Magnet; 400; MHz

The key objective of the following proposal is a replacement of a superconducting magnet for the 400 MHz NMR spectrometer in the Department of Natural Product Biochemistry at the Institute of Biochemistry and Biotechnology of the Martin Luther University Halle-Wittenberg. The Department specializes in protein and natural products biochemistry and, in particular, addresses research questions in the directions of protein (re)folding and stabilization, enzyme design, peptide synthesis and medium engineering with help of NMR spectroscopy. The 400 MHz NMR spectrometer is an essential instrument in the group and of a central importance in the frames of ongoing and planned research projects aimed at elucidating structure-interaction relationships between highly tuneable ionic liquids and complex biomolecules such as peptides and small proteins, as well as studying kinetic of enzymatic reactions. To that end, the high-resolution multidimensional NMR experiments of both solutions and more challenging highly-viscous samples are necessary. With the established high-resolution magic angle spinning NMR toolbox such measurements were possible in the group. In the planned research projects this NMR technique will be further applied to monitor conformational changes and local structure fluctuations of various elongated structured peptides and small proteins upon dissolving them in ionic liquids, as well as to probe the influence of ionic liquids on highly stable structured biomolecules. In addition to structural features NMR spectroscopy will be used to examine dynamics of different ionic liquids as well as biomolecules dissolved in them by exploiting their relaxation properties. Accurate characterization of interactions between ionic liquids and biocatalysts or peptide and protein targets will facilitate the design of application-specific ionic liquids. Finally, NMR spectroscopy will be applied to address cleavage preferences of peptidases targeting various antimicrobial peptides and kinetic parameters of such enzymatic reactions will be derived. In addition the 400 MHz NMR-Spectrometer will be used for routine analysis measurements in MLU Halle.

以下提案的主要目的是在马丁·路德大学哈雷·韦滕伯格（Halle-Wittenberg）的马丁·路德大学（Martin Luther University）生物化学和生物技术研究所替换了自然产品生物化学和生物技术研究所的400 MHz NMR光谱仪的超导磁铁。该部门专门研究蛋白质和天然产品的生物化学，特别是在NMR光谱的帮助下，在蛋白质（RE）折叠和稳定，酶设计，肽合成和中型工程方向上解决了研究问题。 400 MHz NMR光谱仪是该组中的必不可少的工具，并且在正在进行的和计划的研究项目框架的框架中旨在阐明高度可调的离子液体与复杂的生物分子（如肽和小蛋白质）之间的结构相互作用关系，并研究辅助反应的动物学反应。为此，有必要进行两种溶液的高分辨率多维NMR实验和更具挑战性的高粘性样品。通过已建立的高分辨率魔术角旋转NMR工具箱，该组可以进行此类测量。在计划的研究项目中，该NMR技术将进一步应用于监测各种细长结构化肽和小蛋白质的构象变化和局部结构波动，并将其溶解在离子液体中，并探测离子液体对高度稳定的结构化生物分子的影响。除结构特征外，NMR光谱法还将用于检查不同离子液体的动力学以及通过利用其弛豫特性来溶解在其中的生物分子的动力学。离子液体与生物催化剂或肽和蛋白质靶标之间相互作用的准确表征将促进应用特异性离子液体的设计。最后，NMR光谱将应用于靶向各种抗微生物肽的肽酶的裂解偏好，并将得出这种酶促反应的动力学参数。另外，400 MHz NMR光谱仪将用于MLU HALLE中的常规分析测量。