Roles of src tyrosine kinase on neuronal regeneration

src酪氨酸激酶在神经元再生中的作用

基本信息

批准号：
06680734
负责人：
YAMADA Eiji
金额：
$ 0.38万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680734/
关键词：
Neuron Regeneration Src Tyrosine kinase src

项目摘要

In order to obtain information of the roles of src tyrosine kinase on regenerative mechanisms of neurons after axtomy, we immnuhistochemically investigated changes of p60c-src, phosphtyrosine (pTyr), platelet derived growth factor (PDGF) and PDGF receptor. We also studied western blotting analysis of p60c-srcin the dorsal motor nucleus of vagus, nerve, which shows degeneration after axotomy, and the hypoglossal nucleus, which shows regeneration, after nerve transection in adult rats. 1. Increased immunoreactivity of pTyr, which is the reaction product of tyrosine kinase, is a sensitive marker for detection of the activity of tyrosine kinases, was observed in the axotomized neurons in bothe vagal and hypoglossal nuclei. These results indicate that tyrosine kinases concern with axonal reaction. PDGF decreased in the vagal nucleus after axoltomy, while PDGF receptor, which direct associated with Src protein kinase, showed no remarkable change after axotomy. These findings suggest that decrease of PDGF in vagal neurons result in inactivation of Src protein in neurons after axotomy. Src protein itself ashowed no remarkable changes after axotomy in both nuclei, while immunoreactivity for SH3 region of Src was strongly increased in the hypoglossal nucleus after axotomy, while only slightly in the vagal nucleus. These findings suggest that conformational change of Src, which related with activation Src, occurs after axotomy strongly in reganerative hypoglossal neurons, while only slightly in degenerative vagal neurons. Therefore, the activation of Src is thought to concern with neuronal regeneration after axotomy. 2. Western blotting for Src protein showed no significant changes in both vagal and hypoglossal nuclei after axotomy. This result consistent with the immunohistochemical study.

为了获取AXTOMY后SRC酪氨酸激酶在神经元再生机制中的作用的信息，我们通过研究p60c-SRC，磷tyrosine（PTYR），血小板衍生的生长因子（PDGF）和PDGF受体的变化。我们还研究了p60c-srscin的蛋白质印迹分析，神经的背运动核，该分析显示了轴突切开术后的变性，而在成年大鼠中神经横向后的降低核核，该核核显示出了再生。 1。PTYR的免疫反应性升高（酪氨酸激酶的反应产物）是一种敏感的标记，用于检测酪氨酸激酶活性，在轴突化神经元中的神经元中观察到了迷走神经和降临核核的敏感标记。这些结果表明酪氨酸激酶对轴突反应的关注。轴溶后的迷走神经核中PDGF降低，而直接与SRC蛋白激酶有关的PDGF受体在轴切开术后没有显着变化。这些发现表明，迷走神经元中PDGF的降低导致轴突切开术后神经元中的SRC蛋白失活。在两个核中，轴突切开术后SRC蛋白本身均没有显着变化，而轴切开术后降压核中SRC SH3区域的免疫反应却大大增加，而在迷走神经核中仅略微增加。这些发现表明，与激活SRC相关的SRC的构象变化发生在轴切开术中的轴切开术中，而轴突切开术在重新隔离性脑神经元中强烈发生，而仅在退化性迷走神经元中略有略微。因此，轴突切开术后SRC的激活被认为关注神经元再生。 2。SRC蛋白的Western印迹显示轴突切开术后迷走神经和降临核的变化均无显着变化。该结果与免疫组织化学研究一致。