The Regenerative System for Proteins inactivated by Oxidative Stress in Cardiovascular System

心血管系统中氧化应激失活的蛋白质再生系统

• 批准号: 06670755
• 负责人: IKEDA Masaharu
• 金额: $ 1.34万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670755/
• 关键词: Thioredoxin; Thioredoxin Reductase; Glutaredoxin; Oxidative Stress; Regeneration; Vascular Endothelial Cells; Heart; Thioredoxin; Thioredoxin Reductase; Glutaredoxin; Oxidative Stress; Regeneration; Vascular Endothelial Cells; Heart

Protein thiol/disulfide exchanging enzymes such as thioredoxin, thioredocin reductase, glutaredoxin and protein disulfide isomerase were studied as a regenerative (or repair) system for proteins inactivated by oxidative stress using cultured endothelial cells and whole hearts.The results obtained in this study were ;1. Thioredoxin, thioredoxin reductase, glutaredoxin and protein disulfide isomerase were involved in the regeneration reaction of proteins inactivated by oxidative stress in endothelial cells.2. Thioredoxin and glutaredoxin exhibited the different substrate specificity in the regeneration reaction of the oxidatively-damaged proteins.3. The protein levels of thioredoxin and protein disulfide isomerase in vascular endothelial cells and whole hearts were increased by exposure to oxidative stress.4. Human glutaredoxin cDNA was cloned.5. Thioredoxin and thioredoxin reductase were found to be localized in both cytosol and mitochondrial fractions of bovine heart. These enzymes were isolated from each compartment.6. Thioredoxin/thioredoxin reductase system was found to exert a protective effect on the nitric oxidemediated inhibition of mitochondrial respiratory activity.These findings indicated that these protein thiol/disulfide exchanging enzymes, especially thioredoxin/thioredoxin reductase system playd an important role in the cellular antioxidant defense mechanism by functioning as an endogenous regeneration system for oxidatively-damaged proteins.

蛋白质硫醇/二硫化物交换酶，例如硫氧还蛋白，硫代蛋白还原酶，谷物毒素和蛋白质二硫化物异构酶，作为一种再生（或修复）系统，用于使用培养的内皮细胞和整个心脏的氧化应激灭活的蛋白质（或修复）系统。硫氧还蛋白，硫氧还蛋白还原酶，谷蛋白和蛋白二硫化物异构酶参与内皮细胞中氧化应激灭活的蛋白的再生反应。2。硫氧还蛋白和谷蛋白在氧化损伤蛋白的再生反应中表现出不同的底物特异性。3。血管内皮细胞中硫氧还蛋白和蛋白二硫化物异构酶的蛋白质水平通过暴露于氧化应激而增加。4。克隆人类谷歌蛋白cDNA。5。发现硫氧还蛋白和硫氧还蛋白还原酶位于牛心的细胞质和线粒体级分中。这些酶从每个隔室中分离出来。6。发现硫氧还蛋白/硫氧还蛋白还原酶系统对线粒体呼吸活性的一氧化氧化抑制具有保护作用氧化损伤蛋白质的系统。