Generation and metabolism of angiotensins in the vascular wall.

血管壁中血管紧张素的生成和代谢。

基本信息

批准号：
61570436
负责人：
IKEDA Masaharu
金额：
$ 1.41万
依托单位：
Fukuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570436/
关键词：
後肢潅流アンジオテンシンI アンジオテンシンII アンジオテンシン変換酵素血管壁レニンアンジオテンシン系下肢(後肢)還流アンジオテンシン【I】アンジオテンシン【II】血管壁レニン血管壁アンジオテンシン変換酵素

项目摘要

The renin-angiotensin-aldosterone system has been known as one of the most potent controlling system of the blood pressure. The circulating renin-angiotensin system has been thought to be the main source of angiotensins. However, recently there are several reports that the major sites of the formation of angiotensin are peripheral tissues including brain,kidney,adrenal gland,and arterial wall. Full sets of the renin-angiotensin system, i.e.,renin, converting enzyme and renin substrate are reported to exist in those tissues.The purpose of the study was to demonstrate the generation and metabolism of angiotensins using the hindquarter vascular perfusion system and to clarify whether there exists the alternate pathway of angiotensin formation in addition to the classical renin-converting enzyme cascade. Following results were obtained;(1)Hindquarter perfusion system was establishid.(2)Synthetic angiotensin II(10(micrn)g/20ml) was perfused in the perfusing system and angiotensin II was extracted from the perfusate and about 20 % of angiotensin II was recovered.(3)When angiotensin I(100 ng/20 ml) was perfused in the system, about 20 % of angiotensin II was generated. Formation of angiot ensin II was partially inhibited by captopril,a potent converting enzyme inhibitor and more strongly inhibited by captopril plus trasylol,a trypsin-kallikrein inhibitor.(4)When synthetic tridecapeptide renin substrate(10(micrn)g/20 ml) was perfused,angiotensin I and II were recovered from the perfusate. Although captopril could not inhibit the generation of angiotensin II.(5)To check whether the immunoreactive angiotensin II is the octapeptide angiotensin II was also conducted after high performance liquid chromatography.From these results,the vasculature per se plays roles to generate and metabolize angiotensins. In addition, these results also suggest that some enzymes other thana classical reninconverting enzyme system might perticipate in thegeneration or conversion of angiotensins.

肾素 - 血管紧张素 - 醛固酮系统已被称为血压最有效的控制系统之一。循环肾素 - 血管紧张素系统被认为是血管紧张素的主要来源。但是，最近有几份报道称，血管紧张素形成的主要部位是外周组织，包括脑，肾脏，肾上腺和动脉壁。 Full sets of the renin-angiotensin system, i.e.,renin, converting enzyme and renin substrate are reported to exist in those tissues.The purpose of the study was to demonstrate the generation and metabolism of angiotensins using the hindquarter vascular perfusion system and to clarify whether there exists the alternate pathway of angiotensin formation in addition to the classical renin-converting enzyme cascade.获得了以下结果；（1）建立了后肢灌注系统。（2）合成血管紧张素II（10（micrn）g/20ml）在灌注系统中灌注，并从灌注液中提取血管紧张素II，并在血管素II（100 ng/20 ml）中恢复了约20％的血管紧张素II。卡托普利（Captopril）部分抑制Angiot Ensin II的形成，卡托普利（Captopril）是一种有效的转化酶抑制剂，Captopril Plus Trasylol（一种胰蛋白酶-Kallikrein抑制剂）更强烈抑制。（4）当合成三肽肽肾素肾脏底物（10（MICRN）G/20 mL）中均恢复了合成的三蛋白肽肾素蛋白（10（MICRN）GERTUSESINUSENSIMISTEN，II是II的II和II时，尽管卡托普利无法抑制血管紧张素II的产生。（5）检查免疫反应性血管紧张素II是在高性能液相色谱后是否也进行了八磷酸血管紧张素II。从这些结果，脉管本质上可以发挥作用，以产生和代谢血管紧张素。此外，这些结果还表明，某些Thana经典重分酶系统的某些酶可能会在血管紧张素的代代或转化中延伸。