Effects of Exercise on Oxidative Stress and its Protective Mechanism

运动对氧化应激的影响及其保护机制

• 批准号: 11670726
• 负责人: IKEDA Masaharu
• 金额: $ 2.11万
• 依托单位: University of Occupational Health and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670726/
• 关键词: cardiovascular risk factors; aerobic exercise; oxidative stress; oxidative DNA damage; 8-Hydroxy Guanine; MTH-1; lipid peroxidation; TBARS(thiobarbituric acid-reactive substance); 8-ヒドロキシグアニン; DNA修復酵素

The aim of this study was to investigate the cardiovascular effects of the aerobic exercise from the viewpoint of oxygen stress and its protective mechanism.Recent studies reveal that exercise enhances the oxygen consumption which induces the formation of reactive oxygen species and as a result the lipid peroxidation and oxidative DNA damage can be occurred.In this study, we investigate the effects of exercise training on cardiovascular risk factors, lipid peroxidation and oxidative DNA damage.Results were as follows ;(1) Performance of 3-month exercise improved the cardiovascular risk factors.(2) TBARS(thiobarbituric acid-reactive substance), an indicator of lipid peroxidation, significantly reduced after 3-month aerobic exercise, and the reduction of TBARS level positively correlated with frequency of the exercise time.(3) Glutathione peroxidase, an antioxidant enzyme, significantly significantly reduced after 3-month aerobic exercise.(4) TBARS level significantly correlates with waist / hip ratio and Homa's Index, an indicator of insulin resistance.(5) Habitual exercise group showed higher level of 8-Hydroxy Guanine (8-OHdG) than non-habitual exercise group and the 8-OHdG level lowered after exercise load in the non-habitual exercise group but not in the habitual exercise group.(6) On the other hand, mRNA of MTH-1, a repair enzyme, was higher higher level in habitual exercise group than in the non-habitual exercise group and MTH-1 mRNA tended to be induced by exercise load in non-habitual exercise group.These results suggests that aerobic exercise training may protect against oxidative damage of lipid and DNA.

本研究的目的是从氧应激及其保护机制的角度探讨有氧运动对心血管的影响。最近的研究表明，运动会增加耗氧量，从而诱导活性氧的形成，从而导致脂质过氧化本研究探讨运动训练对心血管危险因素、脂质过氧化和氧化DNA损伤的影响。结果如下：（1）3个月的运动改善了心血管危险因素.(2)脂质过氧化指标TBARS（硫代巴比妥酸反应物质）在3个月有氧运动后显着降低，且TBARS水平的降低与运动时间频率呈正相关。（3）抗氧化酶谷胱甘肽过氧化物酶显着降低3个月有氧运动后显着降低。(4)TBARS水平与腰臀比和胰岛素抵抗指标霍马指数显着相关。(5)习惯性运动组的8-羟基鸟嘌呤（8-OHdG）水平高于非习惯性运动组，非习惯性运动组运动负荷后8-OHdG水平降低，而习惯性运动组则没有。（6）另一方面，习惯运动组的修复酶 MTH-1 mRNA 水平高于非习惯运动组，并且 MTH-1 mRNA 往往是由运动负荷诱导的。非习惯性运动组。这些结果表明有氧运动训练可以防止脂质和DNA的氧化损伤。

项目成果

Himeno E: "A weight reduction and weight maintenance program with long-lasting improvementin left ventricular mass and blood pressure"Am J Hypertension. 12. 682-690 (1999)

Himeno E：“减重和体重维持计划，可持久改善左心室质量和血压”Am J 高血压。

Himeno, E.Nishino, K.Nanri, H.Okazaki, T.Komatsu, T.Ikeda, M.: "Evaluation of the Effects of Exercise and a Hypocaloric Diet on Cardiovascular Risk Factors in Obese Subjects."J.UOEH. 23(1). 1-12 (2001)

Himeno、E.Nishino、K.Nanri、H.Okazaki、T.Komatsu、T.Ikeda、M.：“运动和低热量饮食对肥胖受试者心血管危险因素影响的评估。”J.UOEH。

太田雅規,池田正春: "運動の高血圧予防と治療における役割"臨床成人病. 30(9). 1133-1140 (2000)

Masanori Ota、Masaharu Ikeda：“运动在预防和治疗高血压中的作用”《临床成人疾病》30(9)。

Ikeda,M.Nanri,H.: "Evaluation of the Effects of Exercise and a Hypocaioric Diet on Cardiovascular Risk Factors in Obese Subjects."J.UOEH. 23(1). 1-12 (2001)

Ikeda,M.Nanri,H.：“运动和低热量饮食对肥胖受试者心血管危险因素影响的评估。”J.UOEH。

Okazaki T, Himeno E, Nanri H, Ogata H, Ikeda M: "Effects of mild aerbic exercise and a mild hypocaloric diet on plasma leptin concentrations in obese and non-obese women"Clin Exp Pharmacol Physiol. 26. 415-420 (1999)

Okazaki T、Himeno E、Nanri H、Ogata H、Ikeda M：“轻度有氧运动和轻度低热量饮食对肥胖和非肥胖女性血浆瘦素浓度的影响”Clin Exp Pharmacol Physiol。