STUDY ON REFOLDING OF ACTIVE KERATINS

活性角蛋白重新折叠的研究

• 批准号: 06651033
• 负责人: YAMAUCHI Kiyoshi
• 金额: $ 1.28万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06651033/
• 关键词: keratin; protein refolding; wool; hair; biodegradation; 生分解性; タンパク膜; 主体材料

keratins, which are proteins bearing cysteine as much as 10 mole % of total amino acid residues, were extracted from wool and hair as a stable aqueous solution. Chief constituents of the proteins had molecular weight ranging from 40000 to 60000 and an isoelectric point of about 6. The aqueous keratin solution was casted into the clear thin film readily. The film showed high tensile strength and underwent biodegradation by trypsin and other proteases as well as under mouse skin. Both in vitro and in vivo degradations were much slower than the protein film prepared from collagen.TEM and SEM revealed that the keratins in the film were fabricated to a thin thread although a quantitative preparation method has not been established yet. The refolding of the active keratins has been investigated using a purified keratin molecule rather.

角蛋白是一种含有半胱氨酸的蛋白质，占总氨基酸残基的 10 摩尔%，以稳定的水溶液形式从羊毛和头发中提取。蛋白质的主要成分的分子量为40000至60000，等电点约为6。角蛋白水溶液很容易浇铸成透明薄膜。该薄膜表现出高拉伸强度，并可通过胰蛋白酶和其他蛋白酶以及在小鼠皮肤下进行生物降解。体外和体内降解均比胶原蛋白制备的蛋白膜慢得多。虽然尚未建立定量制备方法，但TEM和SEM显示膜中的角蛋白被加工成细线。相反，已经使用纯化的角蛋白分子研究了活性角蛋白的重折叠。

项目成果

K.Yamauchi, A.Yamauchi, T.Kusunoki, A.Khoda, Y.Konishi: "Preparation of stable aqueous solution of keratins, and physicochemical and biodegadational properties of films" Journal of Biomedical Material Research. vol.31, (in press). (1996)

K.Yamauchi、A.Yamauchi、T.Kusunoki、A.Khoda、Y.Konishi：“角蛋白稳定水溶液的制备以及薄膜的物理化学和生物降解特性”《生物医学材料研究杂志》。

K.Yamauchi,A.Khoda: "Preparation and properties of keratin microcapsules" Biomaterials. (in preparation). (1996)

K.Yamauchi，A.Khoda：“角蛋白微胶囊的制备和性能”生物材料。

K.Yamauchi et.al: "Extraction and Cultivation of Mouse L929 Fibroblast Cells on Keratins." J.Biomed.Mat.Res.(in press). (1995)

K.Yamauchi 等人：“在角蛋白上提取和培养小鼠 L929 成纤维细胞。”

K.Yamauchi,A.Yamauchi,T.Kusunoki,A.Khoda,Y.Konishi: "Preparation of stable aqueous solution of keratins,and physicochemical and biodegadational properties of films," Journal of Biomedical Material Research. 31(in press). (1996)

K.Yamauchi、A.Yamauchi、T.Kusunoki、A.Khoda、Y.Konishi：“角蛋白稳定水溶液的制备以及薄膜的物理化学和生物降解特性”，《生物医学材料研究杂志》。

K.Yamauchi, A.Khoda: "Preparation and properties of keratin microcapsules" Biomaterials. (in preparation). (1996)

K.Yamauchi、A.Khoda：“角蛋白微胶囊的制备和性能”生物材料。