STUDIES ON SOME FACTORS RESPOSIBLE FOR CONJUGATED LINOLEIC ACID CONTENT IN FOODS OF RUMINANT ORIGINS

反刍动物食品中共轭亚油酸含量影响因素的研究

基本信息

批准号：
13660269
负责人：
YAMAUCHI Kiyoshi
金额：
$ 2.37万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660269/
关键词：
Conjugated linoleic acid (CLA)c9, t11 isomer of CLA Foods of ruminant origins Concentrate Roughage ration Precursor of c9, t11 isomer Rumen liquor Linoleic acid α -Linolenic acid 反芻家畜出来食品ルーメン内微生物叢共役リノール酸 cis9, trans11 CLA生成飼料

项目摘要

Conjugated linoleic acid (CLA) is a collective term used to describe one or more positional and geometric isomers of linoleic acid. Of the CLA isomers, cis9, trans11 isomer which is found primarily in ruminants amounts as much as 80〜90% of the total CLA content of foods of ruminant origins. The cis9, trans11 isomer of CLA has been shown in animals to have an anticarcinogenic property. It is believed that cis9, trans11 CLA isomer is produced in the rumen as intermediates in the biohydrogenation of dietary linoleic acid to stearic acid. Our works have demonstrated that the cis9, trans11 CLA contents of meat and fat from ruminants fed a relatively high roughage ration is significantly higher than those from ruminants fed a relatively high concentrate ration, with the results suggesting that α -linolenic acid present predominantly in pasture lipid might be involved in the formation of cis9, trans11 CLA.Therefore, crashed corn high in linoleic acid and Italian ryegrass hay high in α -linolenic acid were incubated with rumen liquor culture. The yield of cis9, trans11 CLA from the Italian ryegrass was siginificantly higher than that from the crashed corn. This findings suggested the formation of cis9, trans11 CLA via α -linolenic acid. α -Linolenate was incubated with rumen liquor culture. The formation of cis9, trans 11 CLA from α -linolenate was first observed in the rumen liquor culture, and the pH value of the rumen liquor culture also influenced the metabolism. The results showed that α -linolenate incubated with rumen liquor culture produced three intermediate metabolites of which c9, t11, c15 octadecatrienoic acid might be involved in the formation of cis9, trans11 CLA in the rumen. The results obtained in the present study confirmed for the first time that in additoin to linoleic acid, α -linolenic acid present predominantly in pasture lipid may serve as a potential precursor of cis9, trans 11 CLA in the rumen.

共轭亚油酸（CLA）是一种用于描述一个或多个位置和几何异构体的集体术语，这是9，Trans11异构体。反刍动物的起源。来自反刍动物的反刍动物的肉脂肪的含量，来自反刍动物的浓缩剂的含量很高，结果表明，α-纤维苯甲酸丙糖剂主要在牧场脂质中可能与CIS9的形成有关带有瘤含量的α-晶状体的α-晶状体培养的α型cla是高度的。首先在瘤含量的液化培养物中观察到α-磷酸的CLA，瘤液培养也影响了代谢。在瘤胃中的trans11 cla是当前商店的trans11 cla。