Conjugated linoleic acid of foods originated from ruminant animals and its precursor in feeds

反刍动物食品中的共轭亚油酸及其饲料中的前体

基本信息

批准号：
09660288
负责人：
YAMAUCHI Kiyoshi
金额：
$ 2.11万
依托单位：
Miyazaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660288/
关键词：
Conjugated linoleic acids (CLCs)Determination of 9c, 11t CLA Meat Ruminants Rumen liquor Precursor of 9c, 11t CLA Feed Lipid peroxidation 9cis,11trans Conjugated linoleic acid:9c,11t CLA 濃厚飼料粗飼料前駆脂肪酸リノール酸リノレン酸ルーメン液培養 9c

项目摘要

Conjugated linoleic acids (CLCs) are isomers of linoleic acid which have recently gained importance as one of the biologically active substances in food. Among the various CLA isomers, the 9cis,11trans CLA (9c,11t CLA) found in beef has been shown to have beneficial effects such as antimutagenic and serum cholesterol-lowering acitivities etc.. The objectives of this study are to develop a precise and reproducible method for determining 9c,11t CLA, to determine the 9c,11t CLA contents in various meats by using this developed method, to evaluate the 9c,11t CLA contents in meats from ruminants fed different feeds, to investigate the effects of heating, storage and lipid oxidation on 9c,11t CLA contents in meats, and then to elucidate wethere linolenic acid in feed lipids serves as a precursor of 9c,11t CLA or not .We have devolved a capillary gas liquid chromatographic method for determining 9c,11t CLA in meat. Meat from ruminants contained considerably more 9c,11t CLA than meat from nonruminants. Meat and fat from the high roughage ration-fed ruminants were significantly higher than those from the high concentrate ration-fed ruminants. In addition, the results suggested that the amount of linolenic acid in roughage lipids may affect the 9c,11t CLA content of meats and fats. The results also suggested that 9c, 11t CLA in meat and meat products might be highly stable against heat treatment, storage and lipid oxidation, and that its yield may be quite a small even if 9c,11t CLA should be formed during the progress of lipid peroxidation in meat and meat products. In the incubation of rumen liquor with linolenate. The latter is isomerized to 9c,11t,15c octadecatrienoic acid. The results also suggested that 9c, 11t, CLA might be formed via a rout of hydrogenating 9c,11t,15c octadecatrienoic acid.

共轭亚油酸（CLC）是亚油酸的异构体，最近作为食品中的生物活性物质之一而受到重视。在各种 CLA 异构体中，牛肉中发现的 9cis,11trans CLA (9c,11t CLA) 已被证明具有有益作用，例如抗突变和降低血清胆固醇活性等。本研究的目的是开发一种精确且可靠的 CLA 异构体。测定 9c,11t CLA 的重现性方法，使用该开发的方法测定各种肉类中 9c,11t CLA 的含量，评估饲喂不同饲料的反刍动物肉中9c,11t CLA含量，研究加热、储存和脂质氧化对肉中9c,11t CLA含量的影响，进而阐明饲料脂质中的亚麻酸是否作为9c的前体，是否含有11t CLA。我们开发了毛细管气相色谱法测定肉类中的9c、11t CLA。反刍动物的肉比非反刍动物的肉含有更多的 9c,11t CLA。高粗饲料喂养的反刍动物的肉和脂肪显着高于高精饲料喂养的反刍动物。此外，结果表明粗饲料脂质中亚麻酸的含量可能影响肉类和脂肪中9c、11t CLA的含量。结果还表明，肉和肉制品中的9c、11t CLA对热处理、储存和脂质氧化可能高度稳定，即使在脂质加工过程中形成9c、11t CLA，其产量也可能很小。肉和肉制品中的过氧化。在用亚麻酸孵育瘤胃液中。后者异构化为9c,11t,15c十八碳三烯酸。结果还表明，9c，11t，CLA可能是通过9c，11t，15c十八碳三烯酸氢化途径形成的。