Cloning of Drosophila gene which code for UV-damaged DNA binding protein

编码紫外线损伤 DNA 结合蛋白的果蝇基因的克隆

• 批准号: 03808024
• 负责人: TODO Takeshi
• 金额: $ 1.02万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03808024/
• 关键词: UV damage; DNA repair; Photoreactivating enzyme; Drosophila; ゲルシフト法; DNA結合蛋白

Cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts ((6-4) photoproducts) are the two major classes of cytotoxic mutagenic and carcinogenic DNA photoproducts produced by ultraviolet light irradiation of cells. The phenomenon of photoreactivation, the reduction of the lethal and mutagenic effects of ultraviolet radiation by simultaneous or subsequent irradiation with near ultraviolet or visible light, has been identified in several organisms and in some cases the enzymes that catalyze this process have been characterized in sufficient detail. CPDs are the only known substrate for the photoreactivating enzymes so far analyzed and enzymatic photoreactivation of (6-4) photoproducts has not yet been reported.Using gel shift assay we have identified two protein factors (Factor 1 and Factor 2) which bind specifically to UV-irradiated DNA. After purification we have determined the enzymatic activity of each factor.Factor 2 is a Drosophila homologue of photolyase (which photoreactivate CPDs). Factor 1 is a photoreactivating enzyme of (6-4) photoproduct. This is the first report of such photoreactivating activity specific for (6-4) photoproduct in any organism.

环丁烷嘧啶二聚体（CPD）和嘧啶（6-4）嘧啶酮光产物（（6-4）光产物）是紫外线照射细胞产生的两大类细胞毒性诱变和致癌DNA光产物。光活化现象，即通过同时或随后用近紫外线或可见光照射来减少紫外线辐射的致死和诱变效应，已在多种生物体中得到鉴定，并且在某些情况下，催化该过程的酶已得到足够详细的表征。 CPD 是迄今为止分析的唯一已知的光活化酶底物，并且 (6-4) 光产物的酶促光活化尚未报道。使用凝胶位移测定，我们已经鉴定了两种特异性结合的蛋白质因子（因子 1 和因子 2）紫外线照射的 DNA。纯化后，我们确定了每个因子的酶活性。因子 2 是果蝇光裂合酶（其光再激活 CPD）的同系物。因子1是(6-4)光产物的光活化酶。这是关于任何生物体中 (6-4) 光产物特异的光再激活活性的首次报道。

项目成果

T.TODO et al.: "A new photoreactivating enzyme that specifically repairs ultraviolet light-induced (6-4)photoproducts." Nature. 361. 371-374 (1993)

T.TODO 等人：“一种新的光活化酶，专门修复紫外线诱导的 (6-4) 光产物。”

Takeshi toda and Haruko Ryo: "Identification of cellular factors that recognize UVーdamaged DNA in Drosophila melanogaster" Mutation Research. 273. 85-93 (1992)

Takeshi toda 和 Haruko Ryo：“识别果蝇中紫外线损伤 DNA 的细胞因子”突变研究 273. 85-93 (1992)。

H. Takemori et al: "Chracterization of cellular factors which bind specifically to Ultraviolet light irradiated DNA" J. Radiat. Res. 33. 51 (1992)

H. Takemori 等人：“与紫外线照射的 DNA 特异性结合的细胞因子的特征”J. Radiat。

H.Takemori et al.: "Characterization of cellular factors which bind specifically to Ultraviolet light irradiated DNA." Journal of Radiation Research. 33. 51- (1992)

H.Takemori 等人：“与紫外线照射的 DNA 特异性结合的细胞因子的表征。”

H.TAKEMORO et al.: "Chracterization of cellular factors which bind specifically to Ultraviolet light irradiated DNA." Journal of Radiation Rsearch. 33. 51 (1992)

H.TAKEMORO 等人：“与紫外线照射的 DNA 特异性结合的细胞因子的表征。”