Integrated confocal luminescence spectrometer with spatiotemporal resolution and multiphoton excitation

具有时空分辨率和多光子激发的集成共焦发光光谱仪

• 批准号: 435890300
• 金额: --
• 依托单位: Universität Münster
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/435890300?language=en
• 关键词: Integrated; confocal; luminescence; spectrometer; spatiotemporal

The photophysical investigation of functional materials plays a fundamental role towards an in-depth understanding of luminescent excited state properties at sub- and supramolecular level. For ongoing and future projects at local, national and international level, state of the art spectrally and temporally resolved luminescence analysis techniques need to be combined with the high spatial and temporal resolution of confocal optical microscopy with multiphoton excitation capabilities (including luminescence lifetime imaging microscopy). This will constitute a unique combination that is presently not available in this particular form. Measuring the luminescence of monomeric or aggregated molecular emitters in solutions, homogeneous matrices, fibres, mono- or multilayers, thin films, amorphous powders, crystals, nano- or microparticles with spectral, spatial and temporal resolution at variable temperatures poses a particular challenge due to the highly scattering nature of condensed phases and interfaces. This also applies when the excited state properties of photosensitizers, fluorescent or phosphorescent probes need to be assessed with spectral, spatial and temporal resolution in culture media, bacteria, biofilms, organelles, cells and tissues. In particular, the specific interactions with matrixial, bacterial and eukaryotic (hosts cells or protozoic parasites) components need to be monitored in situ. Moreover, two-photon absorption cross-sections will be determined in homogeneous phases. These challenges will be overcome with the requested instrumentation, which will also enable the assessment of two-photon excitability to reach deep lying structures for bioimaging and phototherapy.

功能材料的光物理研究对于深入了解亚分子和超分子水平的发光激发态特性起着基础作用。对于地方、国家和国际层面正在进行和未来的项目，最先进的光谱和时间分辨发光分析技术需要与具有多光子激发能力的共焦光学显微镜（包括发光寿命成像显微镜）的高空间和时间分辨率相结合。这将构成目前这种特定形式中不可用的独特组合。在不同温度下以光谱、空间和时间分辨率测量溶液、均质基体、纤维、单层或多层、薄膜、无定形粉末、晶体、纳米或微米颗粒中单体或聚集分子发射体的发光提出了特殊的挑战，因为凝聚相和界面的高度散射性质。当需要用培养基、细菌、生物膜、细胞器、细胞和组织中的光谱、空间和时间分辨率评估光敏剂、荧光或磷光探针的激发态特性时，这也适用。特别是，需要在原位监测与基质、细菌和真核生物（宿主细胞或原生寄生虫）成分的特定相互作用。此外，双光子吸收截面将在均质相中确定。这些挑战将通过所需的仪器来克服，这也将能够评估双光子兴奋性以到达深层结构以进行生物成像和光疗。