ANALYSIS ON EXPRESSION OF TUMOR RELATED ANTIGEN AND ITS CLINICAL APLLICATION IN ORAL CANCERS.

口腔癌肿瘤相关抗原的表达及其临床应用分析。

基本信息

批准号：
05671671
负责人：
TSUJI Tatsuo
金额：
$ 1.34万
依托单位：
YAMAGUCHI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671671/
关键词：
IMMUNOHISTOCHEMISTRY FISH p53 PCNA DNA PLOIDY ORAL CANCER Oral camcer Immunohisto chemistry chromosome 17 p53癌抑制遺伝子増殖細胞核抗原 DNA aneuploidy フローサイトメトリー免疫組織化学口腔癌

项目摘要

(1) The immunohistochemical staining of p53 protein was observed in cancer tissues and normal epithelium. Immunoriactivity of p53 and PCNA showed similarities in staining on sqaumous cell carcinoma of the oral cavity, and that p53 score was smaller than PCNA score.(2) The expression of p53 protein was commonly involved in malignant salivary gland tumor and the p53 positive rate was different among various histological types of malignant salivary gland tumors.(3) Numerical aberrations of chromosomes were investigate in oral tumors by fluorescence in situ hybridization, and suggested that numerical aberrations of chromosme occur during the development of malignant tumors of the oral cavity.(4) The expression of tenascin was observed not only tumor cells but also subepithelial border and in various salivary gland tumors. Therefore, it seemed to be implicated in these phenomena.(5) The expression of p53 protein and PCNA was immunohistochemically examined. The immunoreactivity for p53 prote … More in wwas defferent among various malignant salivary gland tumors. p53 protein was more frequently detectable in PCNA positive cases than in negative cases.(6) The cytoplasmic p53 protein expression was observed in the normal cells adjacent to p53 positive carcinomas, but none of the normal cells were positive in the tissue surrounding p53 negative carcinomas. Cytoplasmic expression of p53 protein in salivary gland tissues seems to be correlated with tumorigenesis.(7) The PCNA labeling index was higher in squamous cell carcinomas than in other benign oral lesions. Survival curve of the patients who were negative for p53 was significantly more favorable than for patients who were positive for p53.(8) Polysomy of both chromosomes occurs during the development of salivary gland tumors, and its frequency is increased in adenoid cystic carcinoma as compared to pleomorphic adenoma. A single hybridised signal was much more frequent for chromosome 17 than for chromosome 3. Monosomy of chromosome 17 could possibly be significant in salivary gland tumors.(9) Both of PCNA labeling index and AgNORs counts were significantly higher in squamous cell carcinomas than in leukoplakias. PCNA labeling index apparently increased in leukoplakias with dysplasia and malignant transformed cases.(10) Polysomy of chromosome 3 and 17 occurs during the development of oral tumors. However, the pattern of aneusomy was different among squamous cell carcinomas and salivary gland tumors. Monosomy of chromosome 17 seems to be speciffic in salivary gland tumors. Less

（1）在癌症组织和正常上皮中观察到p53蛋白的免疫组织化学染色。 p53和pCNA的免疫光学性在口腔口腔的细胞癌染色方面显示出相似性，p53评分小于PCNA得分。（2）p53蛋白的表达通常与恶性腺体肿瘤发生恶性肿瘤肿瘤和p53阳性率在各种组织学类型之间具有变性的唾液类型（3个指数）。通过荧光原位杂交在口腔肿瘤中进行了研究，并提出在口腔恶性肿瘤发生期间发生染色体的数值畸变。（4）替纳斯蛋白的表达不仅是肿瘤细胞的表达，而且还观察到了上皮下皮下边界和各种唾液腺肿瘤的表达。因此，它似乎与这些现象有关。（5）对p53蛋白和PCNA的表达进行了免疫组织化学检查。 p53蛋白的免疫反应性…在各种恶性唾液腺肿瘤中，WWA中的免疫反应性更高。在PCNA阳性病例中比在阴性病例中更频繁地检测到p53蛋白。（6）在与p53阳性癌相邻的正常细胞中观察到细胞质p53蛋白表达，但是在p53阴性癌的组织中，没有一个正常细胞呈阳性。唾液腺组织中p53蛋白的细胞质表达似乎与肿瘤发生相关。（7）鳞状细胞癌中PCNA标记指数高于其他良性口服病变。 p53阴性患者的存活曲线比p53阳性的患者明显高得多。（8）在唾液腺肿瘤的发展过程中，两种染色体的拼发发生在唾液腺肿瘤的发育过程中，并且与多余的腺瘤相比，腺体囊性癌中其频率的增加。对于17染色体，单个杂交信号的频率要比染色体3。染色体17的单肌在唾液腺肿瘤中可能具有重要意义。（9）PCNA标记指数和AGNORS指数和Agnors计数在鳞状细胞癌中的数量明显高于白血球癌。 PCNA标记指数在白细胞中显然增加了发育异常和恶性转化病例。（10）在口腔肿瘤的发展过程中发生了3和17的染色体的拼发。然而，在鳞状细胞癌和唾液腺肿瘤中，动脉法的模式不同。 17号染色体的单肌似乎在唾液腺肿瘤中是特异性的。较少的