Analysis of expression of tumor-associated antigen and its clinical application in oral cancers.

口腔癌肿瘤相关抗原的表达分析及其临床应用

• 批准号: 02670898
• 负责人: TSUJI Tatsuo
• 金额: $ 1.6万
• 依托单位: Yamaguchi University Hospital
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670898/
• 关键词: Fluorescence in situ hybridization; Flow cytometry; Proliferating cell nuclear antigen; c-erbB-2 oncogene; Immunohistochemistry; DNA aneuploidy; AgNORs; Oral cancer; 腫瘍マ-カ-; 腫癌関連抗原; 増殖細胞核抗原(PCNA); FISH法; 染色体異常; 増殖細胞マ-カ-; フロ-サイトメトリ-; 免疫組織化学

The numerical aberration of chromosome 17 can be detected by fluorescence in situ hybridization in oral tumors and this correlated with malignant behavior of oral tumors. PCNA immunoreactivity correlated with the differentiation and atypia of the tumor cells in squamous cell carcinoma of the oral cavity. PCNA score was significantly higher in the malignant and pre-malignant lesion, and correlate with the degree of dysplasia in pre-malignant lesions. The mean PCNA score decreased significantly after cancer chemotherapy of squamous cell carcinoma. According to this results, the response of cancer cells to anticancer agents may be estimated by consecutive measurement of PCNA. c-erbB-2 oncoprotein is rarely expressed in malignant salivary gland tumors. However, with a limited number of c-erbB-2 oncoprotein positive cases, it was not possible to correlate the oncogene status with clinical behavior of the tumor. c-erbB-2 did not detected in squamous cell carcinoma, but markedly detected in plemorphic adenoma, and especially in the cells which showed invasive growth activity DNA aneuploidy is not specific for malignant tumors and there was significant difference in DNA index between malignant and benign ledsions. KM-93 antigen detected significantly high level in the patient serum of the salivary gland tumotrs, therefore, it seems that measurement of this antigen is useful method for clinical practice.

可以通过口腔肿瘤的原位杂交来检测17染色体的数值像差，这与口腔肿瘤的恶性行为相关。 PCNA免疫反应性与口腔鳞状细胞癌中肿瘤细胞的分化和非典型性相关。在恶性病变和恶性病变中，PCNA得分明显更高，并且与恶性病变中的发育不良程度相关。鳞状细胞癌的癌症化学疗法后，平均PCNA得分显着降低。根据此结果，可以通过连续测量PCNA来估计癌细胞对抗癌药的反应。 C-ERBB-2癌蛋白很少在恶性唾液腺肿瘤中表达。但是，由于C-ERBB-2癌蛋白阳性病例的数量有限，因此无法将癌基因状态与肿瘤的临床行为相关联。在鳞状细胞癌中未检测到C-ERBB-2，但在群腺瘤中被明显检测到，尤其是在显示出浸润性生长活性DNA脑倍的细胞中，对恶性肿瘤并不具体，并且在恶性和良性累积累积和良性引导之间DNA指数存在显着差异。 KM-93抗原在唾液腺肿瘤的患者血清中检测到显着高水平，因此，该抗原的测量似乎是临床实践的有用方法。

项目成果

田垣内 幸士: "多形性腺腫におけるc-erbB-2癌遺伝子産物の発現" 癌の臨床. 37. 33-35 (1991)

Koji Tagakiuchi：“多形性腺瘤中 c-erbB-2 癌基因产物的表达”癌症诊所。 37. 33-35 (1991)

Tomoyuki Murakami: "Flow cytometry and image analysis for clinical applications" Elsevier Science Publisher B.V., 368 (1991)

Tomoyuki Murakami：“临床应用的流式细胞术和图像分析”Elsevier Science Publisher B.V.，368 (1991)

T.Tsuji,K.Tagaito,K.Sasaki,F.Shinozaki: "cーerbBー2 proto oncogene protein and its correlation with cell differentiation." Anticancer Research.

T.Tsuji、K.Tagaito、K.Sasaki、F.Shinozaki：“cerbB-2 原癌基因蛋白及其与细胞分化的相关性。”

辻 龍雄: "口腔腫瘍におけるc-erbB-2遺伝子産物関連抗原の発現" 日本口腔外科学会雑誌. 37. 944-947 (1991)

Tatsuo Tsuji：“口腔肿瘤中c-erbB-2基因产物相关抗原的表达”日本口腔颌面外科杂志37. 944-947（1991）。

Tatsuo Tsuji, Kohsuke Sasaki, Yuka Kimura, Tomoyuki Murakami, Masamichi Ida, Akihiro Kanekawa, Tsukasa Kuzuyama, Fumihiko Shinozaki: "Flow cytometric analysis of nuclear DNA content in oral lesions" Japanese Journal of Oral and Maxillofacial Surgery. 38.

Tatsuo Tsuji、Kohsuke Sasaki、Yuka Kimura、Tomoyuki Murakami、Masamichi Ida、Akihiro Kanekawa、Tsukasa Kuzuyama、Fumihiko Shinozaki：“口腔病变核 DNA 含量的流式细胞术分析”日本口腔颌面外科杂志。