A Study on the Association between Polymorphism in Dopamine D_4 Receptor Gene and Psychiatric Diseases

多巴胺D_4受体基因多态性与精神疾病相关性研究

• 批准号: 05670797
• 负责人: FUKUDA Masato
• 金额: $ 0.83万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670797/
• 关键词: dopamine receptor,; D_4 receptor gene,; psychiatric disease,; DNA polymorphism,; polymerase chain reaction (PCR); 遺伝子多型; D_4受容体; 多型性

Dopaminergic system are assumed to play an important role in the etiology and the pathophysiology of many psychiatric diseases. Among five types of human dopamine receptors, cDNA polymorphism with repeated sequences has been demonstrated in D_4 receptor gene. The purpose of this syudy is 1) to establish an easy method to determine the polymorphism in D_4 receptor gene using white blood cell DNA,and 2) to investigate the association between the polymorphism and psychiatric diseases.In 1993, amplification of D_4 receptor gene was conducted with polymerase chain reaction (PCR) method using three sets of primers. Specific amplification of D_4 receptor was unsuccessful because many other genes were amplified simultaneously. Survey of the relevant literatures showed that D_4 receptor gene is one of the most difficult one to be amplified by PCR.In 1994, single band in the electrophoresis of PCR products was obtained with many technical improvement and PCR condition adjustment. The band probably represent the intended D_4 receptor gene because the band showed the same electrophoretic Pattern as that of D_4 receptor gene when digested by two different restriction enzymes. Therefore, the D_4 receptor gene is easily amplified with PCR method under the condition determined here. Now, the association between D_4 receptor gene polymorphism and psychiatric diseases is being investigated with the above method.

假定多巴胺能系统在许多精神疾病的病因和病理生理学中起重要作用。在五种类型的人多巴胺受体中，在D_4受体基因中已经证明了具有重复序列的cDNA多态性。该Syudy的目的是1）建立一种简单的方法，使用白细胞DNA确定D_4受体基因中的多态性，以及2）研究多态性和精神疾病之间的关联。在1993年，使用三个集合的聚合酶链反应（PCR）方法对D_4受体基因进行扩增D_4受体基因。 D_4受体的特异性扩增不成功，因为许多其他基因同时被放大。对相关文献的调查表明，D_4受体基因是PCR.in 1994最困难的基因之一，在PCR产物的电泳中获得了单个带，并获得了许多技术改进和PCR条件调整。该频带可能代表了预期的D_4受体基因，因为当通过两种不同的限制酶消化时，带显示了与D_4受体基因相同的电泳模式。因此，在此处确定的条件下，D_4受体基因很容易用PCR方法扩增。现在，通过上述方法研究了D_4受体基因多态性与精神病之间的关联。