Receptors for parathyroid hormone-related protein (PTHrP)

甲状旁腺激素相关蛋白 (PTHrP) 受体

• 批准号: 05670185
• 负责人: MATSUSHITA Hiroshi
• 金额: $ 1.28万
• 依托单位: Okinaka Memorial Institue for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670185/
• 关键词: PTHrP; PTH; calcium ion; parathyroid gland; lymphocyte; cytoskeleton; monoclonal antibody; PTHrP; モノクローナル抗体; 細胞内カルシウム

Parathyroid hormone-related protein (PTHrP) is known to widely distribute in the normal human tissues. The physiological functions of PTHrP in normal tissues are suspected to be a paracrine effector. To reveal intracytoplasmic distribution of PTHrP,we firsts established a monoclonal antibody against PTHrP (clone No. : MMUN-034). There are a few parathyroid cells positive for PTHrP,whereas nodular growth of PTHrP positive cell is frequently found in hyperplastic parathyroid glands. In PTr cells, established rat parathyroid cell line, PTHrP was distributed diffusely with net-work like structure similar to that of vimentin. Intracytoplasmic distribution of PTH significantly changed after the stimulation of recombinant PTHrP in PTr cells. That is, parathyroid hormone in PTr cells localized around the nuclei redistributed to the cell membrane 1 hour after PTHrP stimulation. And PTHrP release from parathyroid cells was inhibited by the extracellular calcium. Therefore, PTHrP in the parathyroid gland is suspected to have an important role in the control of cell growth and PTH secretion.PTHrP was also expressed in both T and B lymphocytes. PTHrP in the activated T lymphocytes was localized in the cytoplasmic snout by which T lymphocytes may contact with B lymphocytes. Thus, PTHrP was suspected to have some important roles in the costimulation system between T and B lymphocytes through the regulation of cell contact.

已知甲状旁腺激素相关蛋白（PTHrP）广泛分布于正常人体组织中。 PTHrP 在正常组织中的生理功能被怀疑是旁分泌效应器。为了揭示PTHrP的胞浆内分布，我们首先建立了抗PTHrP的单克隆抗体（克隆号：MMUN-034）。少数甲状旁腺细胞呈PTHrP阳性，而增生性甲状旁腺常见结节状生长的PTHrP阳性细胞。在建立的大鼠甲状旁腺细胞系PTr细胞中，PTHrP呈弥漫性分布，具有与波形蛋白类似的网状结构。重组PTHrP刺激PTr细胞后，PTH的胞浆内分布发生显着变化。也就是说，PTHrP刺激后1小时，位于细胞核周围的PTr细胞中的甲状旁腺激素重新分布到细胞膜上。甲状旁腺细胞释放PTHrP受到细胞外钙的抑制。因此，甲状旁腺中的PTHrP被怀疑在控制细胞生长和PTH分泌方面具有重要作用。PTHrP在T和B淋巴细胞中也有表达。活化的T淋巴细胞中的PTHrP定位于胞质吻部，T淋巴细胞可通过胞质吻部与B淋巴细胞接触。因此，PTHrP 被怀疑通过调节细胞接触在 T 淋巴细胞和 B 淋巴细胞之间的共刺激系统中发挥一些重要作用。

项目成果

松下 央: "長期透析症例の副甲状腺病変" 診断と治療. 83. 764-765 (1995)

Hiroshi Matsushita：“长期透析病例中的甲状旁腺病变”诊断和治疗。83. 764-765 (1995)。

松下 央: "副甲状腺におけるParathyroid hormone-related proteinの分布と放出調整の機序" 最新医学. 48. 1445-1452 (1993)

松下浩：“甲状旁腺激素相关蛋白的分布和释放调节机制”现代医学48。1445-1452（1993）。

atsushita,H.: "Bloodworth´s Endocrine Pathology (Lechago, J. , Gould, V. E. Ed) , 3rd Ed. ," Williams & Wilkins (in print.), (1995)

atsushita, H.：“Bloodworth 的内分泌病理学（Lechago, J.、Gould, V. E. Ed），第 3 版，”Williams & Wilkins（印刷版），（1995 年）

Matsushita, H.: "Pathology of the Parathyroid Glands. In : Bloodworth's Endocrine Pathology, Third Edition (Lechago, J.and Gould, V.E., Ed)." Wiliams & Wilikins. (in print.). (1995)

Matsushita, H.：“甲状旁腺的病理学。见：Bloodworth 的内分泌病理学，第三版（Lechago, J. 和 Gould, V.E.，Ed）。”

Matsushita, H.: "The pathologic changes of the parathyroid gland in long-term hemodialysis. (in Japanese)" Shindan to Chiryou. 83. 764- (1995)

Matsushita, H.：“长期血液透析中甲状旁腺的病理变化。（日语）” Shindan 到 Chiryou。