The funcation of parathyroid hormone-related protein.

甲状旁腺激素相关蛋白的功能。

• 批准号: 08670231
• 负责人: MATSUSHITA Hiroshi
• 金额: $ 1.41万
• 依托单位: Okinaka Menorial Institute for Medical Researach
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670231/
• 关键词: PTHrP; Parathyriud; Immunohistochemistry; in sitsu Hybridization; mRNA; PCNA; Proliferation; PTH

The localization of parathyroid hormone(PTH)and PTH-related protein(PTHrP)were studied in 42 hyperplastic parathyroid glands in long term hemodialysis cases with immunohistochemistry and in situ hybridization. Results were compared with proliferaative activity(PCNA index : counts of proliferating cell nucleaar antaigen-positive cells/100 cells)on the same section used for PTH/PTHrP immunostaining. Ten normal glands weree studied as the control.In hyperplasia, cells positive for either PTH or PTHrP,or both, were observed immunohisstochemically. The areas expressing PTHrP mRNA were completely coincided with those positive for PTHrP immunohistochemically. Oxyphil or transitional oxyphil cells were consistently positive for PTHrP and showed a tendency for grouping together. The PCNA positive cells were rare in normal glands with index of 0.22(]SY.+-。[)0.09(mean (]SY.+-。[) SEM). Proliferating cell components were significantly increased in hyperplastic cases and indices for PTHrP positive cells were 1.25(]SY.+-。[)0.16 and tose for PTHrP negative cells were 7.8(]SY.+-。[)0.52. Thus, the proliferative activity in PTHrP positive cells was sgnificantly loweer than their PTHrP negative conterparts in secondary hyperplasia(P<0.0001).PTHrP is now mostly suspected to act as an autocrine or a paracrine substance regulating cell growth or differentiation, although it was originally reported as the hypercalcemic substance in malignancies. On the basis of suppressed cell proliferation in PTHrP positive cells, we suggest a functional role of PTHrP as growth suppresser in the human parathyroid glands.

在长期的具有免疫组织化学和原位杂交的长期血液透析病例中，在42个增生性甲状旁腺中的42例增生性甲状旁腺中的42例增生性甲状旁腺的定位和与PTH相关的蛋白质（PTHRP）的定位是在42个增生性甲状旁腺中进行了研究的。将结果与用于PTH/PTHRP免疫染色的相同部分上的增殖活性（PCNA指数：增殖细胞核抗原阳性细胞/100细胞）进行比较。在增生，PTH或PTHRP呈阳性的细胞中，有十个正常的腺体AREE研究。表达PTHRP mRNA的区域与PTHRP免疫组织化学阳性的区域完全相吻合。氧气或过渡氧细胞的PTHRP始终为阳性，并且显示出将组合在一起的趋势。 PCNA阳性细胞在正常腺体中很少见，指数为0.22（]SY。+ - 。[）0.09（平均值（]SY。+ - 。[）SEM）。增生病例中增殖的细胞成分显着增加，PTHRP阳性细胞的指标为1.25（]SY。+ - 。[）0.16，PTHRP阴性细胞的TOSE为7.8（]SY。+ - 。[）0.52。这就是PTHRP阳性细胞中的增殖剂活性低于其继发性增生中的PTHRP阴性计数器（P <0.0001）。现在主要怀疑PTHRP最初被认为是降压细胞生长或分化的旁分泌底物，尽管它最初是在恶性肿瘤中的高钙质药物。在PTHRP阳性细胞中抑制细胞增殖的基础上，我们建议PTHRP作为人甲状旁腺中生长补充剂的功能作用。

项目成果

Matsushita H: "Pathology of the parathyroid glands.In Bloodworth's Endocrine Pathology,3rd Ed." Williams & Wilkins,Baltimore, 709 (1996)

Matsushita H：“甲状旁腺的病理学。见 Bloodworth 的内分泌病理学，第三版。”

Matsushita H,et al.: "Co-secretion of parathyroid hormone and parathyroid hormone-related protein via a regulated pathway in human paratahyroid adenoma cells." Am J Pathol. 150. 861-871 (1997)

Matsushita H 等人：“通过人甲状旁腺腺瘤细胞中的调节途径共同分泌甲状旁腺激素和甲状旁腺激素相关蛋白。”

Asahi Y.: "Establishment of a clonal cell line producing granulocyte colony-stimulating factor and parathyroid hormone-related protein from a lung cancer patient with leukocytosis and hypercalcemia." Jpn J Cancer Res. 87. 451-458 (1996)

Asahi Y.：“从患有白细胞增多和高钙血症的肺癌患者中建立了产生粒细胞集落刺激因子和甲状旁腺激素相关蛋白的克隆细胞系。”

松下 央: "副甲状腺疾患の病理診断の進歩と限界" 日本内分泌学会雑誌. 73. 109 (1997)

Hiroshi Matsushita：“甲状旁腺疾病病理诊断的进展和局限性”日本内分泌学会杂志 73. 109 (1997)。

Matsushita H: "The progress in diagnosis of parathyroid disease.(Japanese)" Nihonnaibunpitsugakkaizasshi. 73. 109 (1997)

松下 H：“甲状旁腺疾病的诊断进展。（日语）”Nihonnaibunpitsugakkaizasshi。