The funcation of parathyroid hormone-related protein.

甲状旁腺激素相关蛋白的功能。

• 批准号: 08670231
• 负责人: MATSUSHITA Hiroshi
• 金额: $ 1.41万
• 依托单位: Okinaka Menorial Institute for Medical Researach
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670231/
• 关键词: PTHrP; Parathyriud; Immunohistochemistry; in sitsu Hybridization; mRNA; PCNA; Proliferation; PTH

The localization of parathyroid hormone(PTH)and PTH-related protein(PTHrP)were studied in 42 hyperplastic parathyroid glands in long term hemodialysis cases with immunohistochemistry and in situ hybridization. Results were compared with proliferaative activity(PCNA index : counts of proliferating cell nucleaar antaigen-positive cells/100 cells)on the same section used for PTH/PTHrP immunostaining. Ten normal glands weree studied as the control.In hyperplasia, cells positive for either PTH or PTHrP,or both, were observed immunohisstochemically. The areas expressing PTHrP mRNA were completely coincided with those positive for PTHrP immunohistochemically. Oxyphil or transitional oxyphil cells were consistently positive for PTHrP and showed a tendency for grouping together. The PCNA positive cells were rare in normal glands with index of 0.22(]SY.+-。[)0.09(mean (]SY.+-。[) SEM). Proliferating cell components were significantly increased in hyperplastic cases and indices for PTHrP positive cells were 1.25(]SY.+-。[)0.16 and tose for PTHrP negative cells were 7.8(]SY.+-。[)0.52. Thus, the proliferative activity in PTHrP positive cells was sgnificantly loweer than their PTHrP negative conterparts in secondary hyperplasia(P<0.0001).PTHrP is now mostly suspected to act as an autocrine or a paracrine substance regulating cell growth or differentiation, although it was originally reported as the hypercalcemic substance in malignancies. On the basis of suppressed cell proliferation in PTHrP positive cells, we suggest a functional role of PTHrP as growth suppresser in the human parathyroid glands.

在长期的甲状旁腺甲状腺中，在42个增生的甲状旁腺中研究了甲状旁腺激素（PTH）和与PTH相关的蛋白质（PTHRP）的定位。 PTHP/PTHRP的PTHRP呈阳性。索引为0.22（]sy。+ - 。）0.09（平均（]SY。+ - 。[）SEM 1.25（]SY。+ - 。 [）PTHRP阴性细胞的0.16和TOSE为7.8（）0.52。自分泌或旁分泌物质调节细胞生长或分化，尽管它最初是根据PTHRP阳性细胞中抑制细胞增殖的高钙血症，但我们建议PTHRP作为人甲状旁腺中生长的功能。

项目成果

Matsushita H: "Pathology of the parathyroid glands.In Bloodworth's Endocrine Pathology,3rd Ed." Williams & Wilkins,Baltimore, 709 (1996)

Matsushita H：“甲状旁腺的病理学。见 Bloodworth 的内分泌病理学，第三版。”

Matsushita H,et al.: "Co-secretion of parathyroid hormone and parathyroid hormone-related protein via a regulated pathway in human paratahyroid adenoma cells." Am J Pathol. 150. 861-871 (1997)

Matsushita H 等人：“通过人甲状旁腺腺瘤细胞中的调节途径共同分泌甲状旁腺激素和甲状旁腺激素相关蛋白。”

Asahi Y.: "Establishment of a clonal cell line producing granulocyte colony-stimulating factor and parathyroid hormone-related protein from a lung cancer patient with leukocytosis and hypercalcemia." Jpn J Cancer Res. 87. 451-458 (1996)

Asahi Y.：“从患有白细胞增多和高钙血症的肺癌患者中建立了产生粒细胞集落刺激因子和甲状旁腺激素相关蛋白的克隆细胞系。”

松下 央: "副甲状腺疾患の病理診断の進歩と限界" 日本内分泌学会雑誌. 73. 109 (1997)

Hiroshi Matsushita：“甲状旁腺疾病病理诊断的进展和局限性”日本内分泌学会杂志 73. 109 (1997)。

Matsushita H: "The progress in diagnosis of parathyroid disease.(Japanese)" Nihonnaibunpitsugakkaizasshi. 73. 109 (1997)

松下 H：“甲状旁腺疾病的诊断进展。（日语）”Nihonnaibunpitsugakkaizasshi。