Neuron circuit analysis on the basis of neuroactive substances and receptors

基于神经活性物质和受体的神经元回路分析

• 批准号: 05454658
• 负责人: MIZUNO Noboru
• 金额: $ 4.22万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454658/
• 关键词: cerebral cortex; striatum; cerebellum; pain; endogenous paincontrol system; opioid receptor; metabotropic glutamate receptor; substance P receptor; グルタミン酸合成酵素; K物質受容体; 排尿中枢; 顎運動; 迷走神経核群; NMDA受容体; P物質; セロトニン; ドパミン; GABA; 網膜

1.An antibody against substance P receptor (SPR) was raised, and distribution of SPR in the central nervous system (CNS) of the rat was examined immunohistochemically. The vast majority of cerebral cortical neurons with SPR were GABAergic non-pyramidal neurons. Striatal neurons with SPR were segregated into somatostatinergic and cholinergic apiny (intrinsic) neurons.2.Distributions of mRNAs for metabotropic glutamate receptors, mGluR2-mGluR7 in the rat CNS were examined by in situ hybridization. Antibodies against mGluR2, and mGluR4 - mGluR8 were raised. Presynaotic localization of m GluRs were immunohistochemically observed light and electron microscopically in the cerebellar cortex (mGluR2 and mGluR4) , layr I of the spinal cord (mGluR7) and olfactory tubercle (mGluR8).3.Two kinds of antibodies against mu-opioid receptor (MOR) were raised, and distribution of MOR in the rat CNS was examined immunohistochemically. Presynaptic localization of MOR was observed light and electron microscopically in the medial terminal nucleus of the accessory optic tract, nucleus of the solitary tract, and layr I of the spinal cord. In layr I of the spinal cord, some axon terminalswith substance P contained MOR.4.Analysis was performed on the local circuitry in the cerebral cortex, striatum and cerebellar cortex, the nociceptive system, the endogeous pain-control system, and masticatory system.

1.产生针对P物质受体（SPR）的抗体，并用免疫组化法检测SPR在大鼠中枢神经系统（CNS）中的分布。绝大多数具有 SPR 的大脑皮层神经元是 GABA 能非锥体神经元。经SPR处理的纹状体神经元分为生长抑素能神经元和胆碱能内源性神经元。2.通过原位杂交检测大鼠中枢神经系统代谢型谷氨酸受体mGluR2-mGluR7 mRNA的分布。产生针对 mGluR2 和 mGluR4 - mGluR8 的抗体。免疫组化光镜和电镜观察小脑皮质（mGluR2和mGluR4）、脊髓第一层（mGluR7）和嗅结节（mGluR8）中m GluRs的突触前定位。 3.两种针对μ阿片受体的抗体（ MOR) 升高，并检查 MOR 在大鼠 CNS 中的分布免疫组织化学。光镜和电子显微镜观察到MOR在副视束内侧终核、孤束核和脊髓第一层的突触前定位。脊髓第一层部分P物质轴突末梢含有MOR。4.对大脑皮层、纹状体和小脑皮层的局部回路、伤害感受系统、内源性疼痛控制系统、咀嚼系统进行分析。

项目成果

李雲慶: "Premptor neurons for trigeminal motor nucleus neurons innervating the jaw-closing and jaw-opening muscles: Differential distribution in the lowor brainstem of the rat" J. Comp. Neurol.356. 563-579 (1995)

李云清：“支配下颌和张开肌肉的三叉神经运动核神经元：大鼠下脑干的差异分布”J. Comp. 563-579（1995）。

大石 仁: "Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex" Neuron. 13. 55-66 (1994)

Hitoshi Oishi：“大鼠小脑皮质中代谢型谷氨酸受体 mGluR2 和 mGluR3 的免疫组织化学定位”Neuron。13. 55-66 (1994)

赤沢 千尋: "Expression of mRNAs of L-AP4 sensitive metal-otropic glutamute receptors(mGluR4,mGluR6,mGluR7)in the rat retina" Neurosci Lett.(印刷中). (1994)

Chihiro Akazawa：“大鼠视网膜中 L-AP4 敏感金属谷氨酸受体（mGluR4、mGluR6、mGluR7）的 mRNA 表达”Neurosci Lett（出版中）。

重本 隆一: "Immunohistochemical localization of a metal-otropic glutamate receptor,mGluR5,in the rat brain" Neurosci.Lett.163. 53-57 (1993)

Ryuichi Shigemoto：“大鼠大脑中金属型谷氨酸受体 mGluR5 的免疫组织化学定位”Neurosci.Lett.163 (1993)。

金子 武嗣: "Glutamate-synthesizing enzymas in GABAergic neurons of the neocortex: a double immunofluorescence study in the rat" Neuroscience. 61. 839-849 (1994)

Takeshi Kaneko：“新皮质 GABA 能神经元中的谷氨酸合成酶：大鼠的双重免疫荧光研究”《神经科学》61. 839-849 (1994)。