Cytogenetics

细胞遗传学

基本信息

批准号：
7714122
负责人：
THEODORE G KRONTIRIS
金额：
$ 6.13万
依托单位：
BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2012-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7714122
关键词：
Acute Myelocytic Leukemia Adoptive Immunotherapy Aneuploidy Basic Science Biological Assay Biological Models Budgets Cancer Center Cancer Center Support Grant Cell Line Cells Cities City of Hope Comprehensive Cancer Center Clonality Core Facility Cytogenetics Cytogenetics Shared Resource DNA Probes Defective spinal cord development Detection Development Dose Dysmyelopoietic Syndromes Equipment Fees Fluorescence Fluorescent in Situ Hybridization Freezing Funding Gene Mutation Genes Genomic Instability Genotype Hematopoiesis Image Analysis Immunophenotyping Interphase Investigation Karyotype determination procedure Laboratories Malignant Neoplasms Maps Molecular Molecular Cytogenetics Molecular Genetics Monitor Neoplasms Patient Selection Peer Review Phenotype Polymerase Chain Reaction Productivity Publications Reporting Research Research Personnel Residual Tumors Resource Sharing Role Scientific Advances and Accomplishments Scientist Services Signal Transduction Solid Neoplasm Source Southwest Oncology Group Spectral Karyotyping Technical Expertise Technology Testing Tissues Training Translational Research Treatment Protocols base cancer cell cancer genetics gene therapy genetic technology member mouse model nucleic acid localization programs quality assurance tissue/cell culture tool

项目摘要

The Cytogenetics Core Facility provides cytogenetics, molecular cytogenetics, and molecular clonality assays to City of Hope Cancer Center (CC) investigators. Primary services of this facility include: cell line characterization and verification of integrity, tissue (cell) culturtng, solid tumor cytogenetics, assays for genomic instability, fluorescence photomicroscopy, and fluorescence in situ hybridization (FISH) studies for aneuploidy detection, gene tracking, specific gene alterations, genotype/phenotype targeted FISH (T-FISH) analyses for residual disease testing, and gene integration/mapping studies. Spectral karyotyping or 24- color karyotyping, 20-color karyotyping for mouse model systems, and FICTION (fluorescence immunophenotyping and interphase cytogenetics as a tool for investigation of neoplasms) or T-FISH (sequential phenotypic followed by genotype analysis) are also available. Tyramide signal amplification FISH is used for the cellular localization of nucleic acids when target levels are suspected to be low; e.g., in gene or adoptive immunotherapy studies. Due to CC members' needs, we expanded our services to provide (1) clonality testing to monitor for clonal hematopoiesis, a precursor of therapy-related acute myelogenous leukemia/myelodysplasia observed in some patjents who received dose-intensive regimens; and (2) Bacterial Artificial Clones (BAG) arrays (aCGH) for comprehensive molecular karyotyping. We also provide imaging analysis training for scientists who wish to screen their own studies or who require extensive usage. Finally, this core is also the reference FISH core laboratory for the SWOG cytogenetics program. During the most recent 12-month reporting period, the Cytogenetics shared resource was used by 18 Cancer Center members from all 5 Research Programs. Peer-reviewed usage represented 97% of total usage. Annual budget for this core is $292,811, of which 22% is institutional funding, 22% is user fees. 26% is from other sources, and 29% is requested from the CCSG.

细胞遗传学核心设施提供细胞遗传学、分子细胞遗传学和分子克隆学向希望之城癌症中心 (CC) 研究人员进行检测。该设施的主要服务包括：细胞系完整性的表征和验证、组织（细胞）培养、实体瘤细胞遗传学、分析基因组不稳定性、荧光显微术和荧光原位杂交 (FISH) 研究非整倍体检测、基因追踪、特定基因改变、基因型/表型靶向 FISH (T-FISH) 残留疾病检测分析和基因整合/绘图研究。光谱核型分析或 24- 彩色核型分析、小鼠模型系统的 20 色核型分析以及 FICTION（荧光免疫表型分析和间期细胞遗传学作为肿瘤研究的工具）或 T-FISH （依次进行表型分析和基因型分析）也是可用的。酪酰胺信号放大当怀疑目标水平较低时，FISH 用于核酸的细胞定位；例如，在基因或过继免疫治疗研究。由于CC会员的需求，我们将服务范围扩大到提供（1）克隆性测试以监测克隆性造血，这是治疗相关急性发作的前兆在一些接受剂量密集治疗的患者中观察到骨髓性白血病/骨髓增生异常； (2) 用于全面分子核型分析的细菌人工克隆 (BAG) 阵列 (aCGH)。我们也为希望筛选自己的研究或需要的科学家提供成像分析培训广泛使用。最后，该核心也是 SWOG 细胞遗传学的参考 FISH 核心实验室程序。在最近 12 个月的报告期内，使用了细胞遗传学共享资源由来自所有 5 个研究项目的 18 名癌症中心成员组成。同行评审的使用占 97% 总使用量。该核心的年度预算为 292,811 美元，其中 22% 为机构资金，22% 为用户资金费用。 26% 来自其他来源，29% 是向 CCSG 请求的。