Morphological studies on neuronal and molecular features of sensory input-controls

感觉输入控制的神经元和分子特征的形态学研究

基本信息

批准号：
12680743
负责人：
MIZUNO Noboru
金额：
$ 2.3万
依托单位：
TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680743/
关键词：
spinal nucleus of trageminal mesencephalic uncleus of trrigeminal nerve raphe nuclei NK1 receptor GABA receptor calcium-binding protein protein kinase C pain 三叉神経感覚核 P物質 P物質受容体セロトニン GABA グリシン

项目摘要

1.The EM study in the rat medullary dorsal horn (MDH) suggested that substance P (SP) released from axon terminals might not only act on NK1 receptors facing SP-containing axon terminals but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act NK1 receptors at some distances from the synaptic sites.2.Co-existence of calcium-binding protein in neurons of the rat medullary dorsal horn was examined by double immunofluorescence histochernistry.3.Neuronal cell bodies in the mesencephalic nucleus of teigeminal nerve (MNT) of the rat were indicated to be in synaptic contact with GABAergic axon terminals and to express functional GABA_<A>receptors but not functional GABA_<B>receptors. Seretoninergic innervation of MNT neurons by the raphe nuclei were also observed.4.SP-containing primary afferent fibers were found to be in synaptic contact with GABAergic and glycinergic neurons in laminae I and II of the rat MDH.5.In slice perparations, correspondence between morphological features and electrical membrane properties of rat MDH neurons were examined in slice preparations by intracellular recording and biocytin-injection combined with histochemical and immunohistochemical staining techniques. The intrinsic MDH neurons were classified into 4 groups.6.Some neurons in the rat dorsal raphe nucleus (DR) were found to project simultaneously to the striatum and the raphe magnus nucleus/gigantocellular nucleus alpha region by axon collaterals. Correspondence between morphological features and electrical membrane propeties of DR neurons were also examined in slice perparations by intracellular recording and biocytin-injection combined with imrnunohistochemistry.7.It was indicated in the rat that protein kinase C gamma was expressed in many nociception-related MDH neurons.

1.在大鼠髓质角（MDH）中进行的EM研究表明，从轴突末端释放的物质不仅在面向含Sp的轴突末端的NK1受体上起作用，而且在细胞外液中的弥漫性比对距离进行弥漫。突触裂fto clett在距突触部位的某些距离处对ACT NK1受体。2。通过双免疫荧光组化的大鼠髓质背角神经元中钙结合蛋白的钙存在性。大鼠的神经（MNT）被表明与GABA能轴突末端接触并表达功能性GABA__ <a>受体，但不是功能性GABA__ <b>受体。还观察到MNT神经元通过Raphe核神经支配。4。在laminae I和II中，含有SPSP的一级一级传入纤维与Gabaergic和Gabaergic和Gabaergic和Glycinagic神经元的突触接触。通过细胞内记录和杀生物细胞素的注射结合组织化学和免疫组织化学染色技术，在切片制剂中检查了大鼠MDH神经元的形态特征和大鼠MDH神经元的电膜特性。固有的MDH神经元分为4组。6。大鼠背侧raphe核（DR）中的一些神经元同时通过轴突旁边的轴突同时投射到纹状体和raphe magnus核/巨大细胞核alpha区域。还通过细胞内记录和生物汤蛋白注射与imrnunoystrosichemistry.7。。