Anti-aging effects of natural antioxidants on senescence accelerated mouse (SAM)

天然抗氧化剂对加速衰老小鼠（SAM）的抗衰老作用

基本信息

批准号：
62860010
负责人：
KAWAKISHI Shunro
金额：
$ 4.1万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62860010/
关键词：
SAM sesame sesaminol vitamin E peroxidized lipids catechin anti-aging effects ビタミンE 老化(SAM)マウス抗酸化性

项目摘要

To investigate the effects of antioxidants in foods on the aging of biological function, the senescence accelerated mouse (SAM) were used through this study as a test animal. First, the studies on the condition of breeding, dietary intake which contains natural antioxidants and on the morphological and biochemical evaluation for SAN fed the diets containing antioxidants were done and the following results were obtained. Though this study, sesame seed products and sesaminol isolated from sesame oil were used as antioxidants.1) By the breeding in long time with the diets added the crushed sesame seeds, the peroxidation of lipids and the increases of lipofuscin in live were effectively depressed in comparison with control group without crushed sesame.2) Peroxidized lipids in liver and plasma, hemolysis, and pyruvate kinase activity in plasma were markedly increased by the breeding with tocopherol deficient diets, however, the addition of sesame products (crushed sesame seeds and oils) depressed to same level with tocopherol containing diets.3) The increase of peroxidized lipids in liver of SAM with tocopherol deficient diets was depressed by the addition of sesaminol, a potent antioxidative lignan in sesame oil, to above diets (0.1%). The dosage of carbon tetra chloride to SAM also induces lipid peroxidation in its liver and plasma, but it was also suppressed to ordinary level by the addition of sesaminol to diets.4) On the same experiments above 2) and 3) using tocopherol deficient diets, a little depression of lipid peroxidation in liver, kidney and plasma was observed by the addition of catechin and its analogues from tea leaf to diets.

为了研究抗氧化剂对食物对生物功能衰老的影响，通过这项研究将衰老加速的小鼠（SAM）用作测试动物。首先，对含有天然抗氧化剂的饮食摄入以及对SAN的形态和生化评估的研究进行了研究，并获得了含有抗氧化剂的饮食，并获得了以下结果。尽管这项研究，从芝麻油中分离出的芝麻种子产品和芝麻醇被用作抗氧化剂。1）长期以来繁殖随着饮食的添加而添加碎芝麻种子，脂质的过氧化，脂质的过氧化和生物中脂肪蛋白在活中的增加有效地抑制与对照组相比，没有压碎的芝麻。2）肝脏和血浆中的过氧化脂质，血液溶解和丙酮酸激酶活性在血浆中的活性显着增加，从而通过育种饮食不足，但是添加了芝麻（压碎的芝麻种子和油和油脂）与含有饮食的生育酚相同的水平。3）通过添加芝麻醇（一种有效的芝麻油中有效的抗氧化木lignan），将SAM肝脏中过氧化的脂质增加抑制了饮食中的饮食，从而抑制了上述饮食（0.1％）。将碳氯化碳氯化碳剂量的剂量也诱导其肝脏和血浆中的脂质过氧化，但也通过将芝麻醇添加到饮食中抑制了普通水平。4），通过添加儿茶素及其类似物从茶叶到饮食来观察到肝脏，肾脏和血浆中脂质过氧化的少量抑郁。