Study of liposomes as drug-carrier.

脂质体作为药物载体的研究。

基本信息

批准号：
58870066
负责人：
SAITO Kazuhisa
金额：
$ 3.01万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1983
资助国家：
日本
起止时间：
1983 至 1985
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-58870066/
关键词：
drug-carrier liposome hepatoma salmonellosis diabetes missile therapy アドリアマイシン経口インスリン

项目摘要

1. Liposome-entrapped streptomycin(L-SM) was very effective against salmonellosis in mice, although free SM was ineffective. Entrapment in liposomes markedly reduced the acute toxicity of SM. Large amounts of SM accumulated in the liver and spleen after i.v. injection of L-SM, and growth of salmonella in these organs was effectively inhibited.2. Adriamycin(Ad) entrapped in anti-human <alpha> -fetoprotein(AFP) monoclonal antibody-conjugated liposomes bound selectively to AFP-producing human hepatoma cells and inhibited their growth in vitro, and also inhibited the growth of the human hepatoma implanted under the skin or the capsule of the liver of nude mice as assessed from the tumor-weight and blood concentraion of AFP. Entrappment in liposomes reduced the toxicity of Ad.3. Intragastric administration of liposome-entrapped insulin reduced blood sugar in only one out of four streptozotocin-induced diabetic mice, and no relation was observed between the decrease of blood sugar and blood concentration of insulin. Intraperitoneal injection of liposome-entrapped insulin reduced blood sugar in four out of six diabetic mice, and in this case the decrease of blood sugar coincided with blood concentration of insulin. These results indicate that improvement in the preparation of liposomes should be necessary to achieve successfull oral administration of insulin for therapy of diabetes mellitus.

1。脂质体结合链霉素（L-SM）在小鼠中对沙门氏菌病非常有效，尽管自由SM无效。脂质体的夹带显着降低了SM的急性毒性。静脉注射后，大量的SM积聚在肝脏和脾脏中在这些器官中注射L-SM和沙门氏菌的生长有效抑制2。阿霉素（AD）被困在抗人类<Alpha> -Fetopopoin（AFP）单克隆抗体偶联的脂质体中，选择性地结合到与AFP产生的人类肝癌细胞的选择性结合，并在体外抑制其生长，并抑制植入人类HEPATOMA的生长根据AFP的肿瘤体重和血液浓度评估的裸鼠肝脏的皮肤或囊囊。脂质体的陷入障碍降低了AD的毒性3。在四分之四的链霉菌素诱导的糖尿病小鼠中，脂质体结合胰岛素的胃内给药减少了血糖，并且在血糖降低与胰岛素的血液浓度之间没有观察到任何关系。在六只糖尿病小鼠中，腹膜内注射脂质体结合的胰岛素减少了血糖，在这种情况下，血糖的减少与血液浓度胰岛素相一致。这些结果表明，要改善脂质体的制备，对于成功地给予胰岛素治疗糖尿病的胰岛素治疗应该是必要的。