Development of angiogenic therapy with human Muse cells

人类 Muse 细胞血管生成疗法的发展

• 批准号: 25670749
• 负责人: YOSHIMURA Kotaro
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670749/
• 关键词: 創傷治癒; 幹細胞; 糖尿病; 皮膚潰瘍; 多能性幹細胞

SSEA-3+ multipotent mesenchymal cells (Muse cells) were isolated from cultured human adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating diabetic skin ulcers was evaluated. Muse-rich ASCs showed upregulated and downregulated pluripotency and cell proliferation genes, respectively, compared to Muse-poor counterpart. Skin ulcers were generated in SCID mice with type 1 diabetes, which showed delayed wound healing compared to non-diabetic SCID mice. Treatment with Muse-rich cells significantly accelerated wound healing compared to treatment with Muse-poor cells. Transplanted cells were integrated into the regenerated epidermis and dermis as epidermal keratinocytes and vascular endothelial cells, respectively. However, they were not detected in the surrounding intact regions. Thus, the selected population of ASCs has greater therapeutic effects to accelerate impaired wound healing associated with type 1 diabetes.

从培养的人脂肪组织来源的干细胞/基质细胞 (hASC) 中分离出 SSEA-3+ 多能间充质细胞（Muse 细胞）并进行表征，并评估其治疗糖尿病皮肤溃疡的治疗潜力。与缺乏 Muse 的 ASC 相比，富含 Muse 的 ASC 分别表现出上调和下调的多能性和细胞增殖基因。患有 1 型糖尿病的 SCID 小鼠出现皮肤溃疡，与非糖尿病 SCID 小鼠相比，伤口愈合延迟。与缺乏 Muse 的细胞相比，富含 Muse 的细胞治疗显着加速了伤口愈合。移植的细胞分别作为表皮角质形成细胞和血管内皮细胞整合到再生的表皮和真皮中。然而，在周围完整的区域中没有检测到它们。因此，选定的 ASC 群体对于加速与 1 型糖尿病相关的受损伤口愈合具有更大的治疗效果。