Influence of ionizing irradiation on tissue-resident stem cells

电离辐射对组织驻留干细胞的影响

基本信息

批准号：
24659780
负责人：
YOSHIMURA Kotaro
金额：
$ 2.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
起止时间：
2012 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659780/
关键词：
再生医学放射線幹細胞創傷治癒放射線障害

项目摘要

Introduction: It remains unclear how ionizing radiation damages healthy skin and hair follicles in a short and long term.Methods: C57BL/J6 mouse (8 weeks old) were distributed to three groups (control: CTR,10Gy once irradiation: 10Gy×1, 5Gy twice irradiations: 5Gy×2). After hair epilation, the groin area was radiated. At 0, 1, 2, and 4 weeks, 3, 6 months after irradiation, the skin samples were evaluated histologically and macroscopically.Results: At 1, 2, and 4 weeks, the epidermis was thicker in 10Gy×1 than CTR and 5Gy×2,without increasing the layer number of epidermal keratinocytes. The number of capillaries in dermis was fewer in 5Gy×2 and 10Gy×1 than CTR at 1 and 2 weeks. At 4 weeks, the the dermis was thicker in CTR than 5Gy×2 and 10Gy×1. At 6 months, the subcutaneous fat tissue was thinner in 5Gy×2 than 10Gy and CTR. At 3 and 6 months, capillaries in groin fat of5Gy×2 and 10Gy×1 appeared to be fewer than thoes of CTR and the size of adipocytes became smaller in 5Gy×2. Many of hair shafts disappeared at 1 week, followed by gradual regrow of hairs by 3 months. The number and size of hair remained decreased in 5Gy×2 and 10Gy×1 than CTR at 6 months when hairs were restored and the gray hairs were macroscopically observed.Discussions/Conclusions: In short term (1 to 4 weeks), the number of hair follicles andcapillaries decreased and the dermis became thinner because of acute radiation injury. In long term (3 to 6 months), tissue damage was generally more severe in 5Gy×2 than 10Gy×1. These data provide clinical insights for minimizing short and long term radiation injuryand revitalizing the radiated ischemic and fibrous tissue.

简介：目前尚不清楚电离辐射如何在短期和长期内损害健康的皮肤和毛囊。方法：C57BL/J6小鼠（8周龄）分为三组（对照组：CTR，10Gy一次照射：10Gy×1， 5Gy两次照射：脱毛后，在0、1、2、4处照射腹股沟区域。照射后4周、3、6个月，对皮肤样本进行组织学和肉眼评价。结果：1、2、4周时，10Gy×1的表皮比CTR和5Gy×2的表皮更厚，且层数不增加。 5Gy×2和10Gy×1时真皮内毛细血管数量少于CTR。第1周和第2周，CTR时真皮比5Gy×2和10Gy×1厚，第6个月时，5Gy×2比10Gy和CTR时皮下脂肪组织更薄。，5Gy×2和10Gy×1腹股沟脂肪中的毛细血管似乎比CTR要少，并且脂肪细胞的大小5Gy×2时，许多毛干在1周时消失，随后3个月时毛发逐渐重新生长。5Gy×2和10Gy×1时，毛发的数量和大小仍比6个月时的CTR减少。恢复，肉眼可见白发。讨论/结论：短期内（1～4周）毛囊和毛细血管数量减少，毛细血管数量减少。长期（3至6个月），真皮因急性放射损伤而变薄，5Gy×2的组织损伤通常比10Gy×1更严重，这些数据为最大限度地减少短期和长期放射损伤和恢复放射损伤提供了临床见解。缺血组织和纤维组织。