Development of live cell imaging experimental system in babesia parasite

巴贝虫寄生虫活细胞成像实验系统的研制

• 批准号: 24658242
• 负责人: KAWAZU Shin-ichiro
• 金额: $ 2.66万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658242/
• 关键词: 獣医学; 感染症; バベシア; ライブイメージング; 遺伝子改変原虫

The system for stable expression of green fluorescent protein (GFP) in babesia parasite was developed and the GFP-expressing parasite was observed through time-lapse video microscopy. (1) Stable expression of GFP in B. bovis using the WR99210/hDHFR selection system was achieved. The expression cassette was applied for the development of gene targeting (knockout) technique and the knockout parasite was established for the first time. (2) The gliding motility of B. bovis merozoites was revealed for the first time by in vitro time-lapse video microscopy of the GFP-expressing parasite. In vivo infection model of the parasite with SCID mice, with which a part of the circulating erythrocyte had been replaced with bovine cells, was also developed to investigate the gliding motility of the parasite in detail through time-lapse video microscopy.

开发了巴贝虫寄生虫中绿色荧光蛋白（GFP）稳定表达的系统，并通过延时视频显微镜观察了表达 GFP 的寄生虫。 (1)利用WR99210/hDHFR选择系统实现了GFP在B. bovis中的稳定表达。该表达盒用于开发基因打靶（敲除）技术，并首次建立了敲除寄生虫。 (2)通过表达GFP的寄生虫的体外延时视频显微镜首次揭示了牛芽孢杆菌裂殖子的滑行运动。还开发了 SCID 小鼠体内寄生虫感染模型，其中部分循环红细胞已被牛细胞取代，以通过延时视频显微镜详细研究寄生虫的滑行运动。

项目成果

Babesia bovisチオレドキシンペルオキシターゼBbTPx-1ノックアウト原虫は活性窒素種負荷に対する感受性が上昇する

牛巴贝斯虫硫氧还蛋白过氧化物酶 BbTPx-1 敲除原生动物对活性氮负荷的敏感性增加

• 发表时间: 2013
• 作者: 麻田正仁;Carlos Suarez;薄井美帆;後藤康之;横山直明;井上昇;矢幡一英;金子修;河津信一郎
• 通讯作者: 河津信一郎

Study on gliding motility of Babesia bovis merozoites using bioimaging analysis.

利用生物成像分析研究牛巴贝虫裂殖子的滑行运动。

• 发表时间: 2014
• 作者: Asada;M.;and Kawazu;S.
• 通讯作者: S.

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Study on gliding motility of Babesia bovis merozo ites using bioimaging analysis

牛巴贝虫早幼虫滑翔运动的生物成像研究

• 发表时间: 2014
• 作者: Asada;M.;and Kawazu;S
• 通讯作者: S

Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy.

• DOI: 10.1371/journal.pone.0035227
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Asada M;Goto Y;Yahata K;Yokoyama N;Kawai S;Inoue N;Kaneko O;Kawazu S
• 通讯作者: Kawazu S