Dissecting the genetic basis of root vigor : applications for improving rice for a changing world

剖析根系活力的遗传基础：在不断变化的世界中改良水稻的应用

• 批准号: 19F19709
• 负责人: Wissuwa Matthias
• 金额: $ 1.41万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-07-24 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19709/
• 关键词: phosphorus efficiency; crossing population; root-vigor; QTL; chromosome 4; P-stress; RNA sequencing

During my JSPS Fellowship, I conducted research focusing on understanding phosphorus(P) efficiency mechanism in rice. My experimental material included two rice breeding parents and their crossing population contrasting in root-vigor related traits and P efficiency in low P soils. I carried out quantitative trait loci (QTL) analysis to determine the underlying genetic basis of the P-efficient root traits. My analysis identified a QTL on chromosome 4 potentially contributing to elongating root length in low P conditions. I also followed up how these contrasting P efficient rice genotypes responded to when P availability was experimentally reduced. My results revealed that the high P-efficient genotype rapidly responded to low P availability by expressing the P-stress related genes.

在JSP奖学金期间，我进行了研究，重点是了解水稻中的磷（P）效率机制。我的实验材料包括两个水稻育种父母及其与根相关性状的交叉种群对比，而在低P土壤中的P效率。我进行了定量性状基因座（QTL）分析，以确定P效率根特征的基本遗传基础。我的分析确定了在4染色体上的QTL可能导致在低P条件下延长根长度的QTL。我还跟进了这些对比鲜明的P有效的水稻基因型如何在实验降低P时响应P。我的结果表明，高P效率的基因型通过表达与P压力相关的基因来迅速响应较低的P可利用率。