イネのリン吸収における根圏域および根圏微生物の影響

根际及根际微生物对水稻磷吸收的影响

• 批准号: 14F04755
• 负责人: Wissuwa Matthias
• 金额: $ 1.34万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2014
• 资助国家: 日本
• 起止时间: 2014-04-25 至 2017-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14F04755/
• 关键词: aerenchyma; P deficiency; DJ; Nerica4; biomass accumulation; RNA-Seq; リン吸収; 微生物叢; 根の吸収効率; バイオマス; 根圏微生物; 土壌微生物; イネのリン吸収; 遺伝子同定; リン獲得効率

Measurements of root porosity suggested that formation of aerenchyma is more rapid under P deficiency and that this is particularly strong for the genotype with higher tolerance to P deficiency. These results were confirmed using more detailed cytological examinations in combination with image analysis tools. The tolerant genotype DJ had a higher proportion of aerenchymatic tissue compared to Nerica4 and this further increased under P deficient conditions. Aerenchyma formation is thus one of the candidate mechanisms that may explain why DJ is generally performing better under low-input conditions, and specifically why it is capable of utilizing P more efficiently in biomass accumulation when P is the limiting factor.Analysis of gene expression patterns for three gene families known to be involved in aerenchyma formation produced suggestive rather than conclusive data. It identified a few candidate genes differentially expressed in contrasting genotypes. Further studies are needed to follow up on this. A manuscript describing his work on aerenchyma is nearing completion and will be submitted for review soon.Furthermore, his assistance with the bioinformatics analysis of the RNA-Seq dataset was instrumental in completing the study of Dr. Nanda. This work is now under review.

根孔隙率的测量表明，在P缺乏症下，气源的形成更快，对于P缺乏症的耐受性较高的基因型尤其强。使用更详细的细胞学检查与图像分析工具结合使用，证实了这些结果。与NERICA4相比，耐受性基因型DJ具有更高的气氛组织比例，并且在p不足条件下这进一步增加了。因此，气氛形成是一种候选机制之一，可以解释为什么DJ通常在低输入条件下表现更好，特别是为什么当P是限制因子时，它能够在生物量积累中更有效地利用P。在限制因子的分析中，基因表达模式的分析是针对三种基因家族的基因表达模式的分析，而已知的三种基因族与Eernectyma形成有关的基因表达模式，而不是结论性的数据。它确定了一些在对比基因型中差异表达的候选基因。需要进一步的研究来跟进这一点。描述他在Aerechyma工作的手稿即将完成，并将很快提交审查。Furthermore，他对RNA-SEQ数据集的生物信息学分析的协助有助于完成Nanda博士的研究。这项工作正在审查中。

项目成果

Lysigenous aerenchyma development in rice roots under Phosphorus deficiency in two contrasting genotypes.

两种对比基因型缺磷条件下水稻根部溶源性通气组织的发育。

• 发表时间: 2017
• 期刊: - (投稿準備中)
• 作者: Milos Havelka;He Zhou;Masaki Ichimura;Seishi Hagihara;Takafumi Fujimoto;Etsuro Yamaha;Shinji Adachi;Katsutoshi Arai;Milos Havelka;Pujol V and Wissuwa M
• 通讯作者: Pujol V and Wissuwa M

Patterns of stress response and tolerance based on transcriptome profiling of rice crown tissue under Zn deficiency.

基于缺锌条件下水稻冠部组织转录组分析的胁迫反应和耐受性模式。

• 发表时间: 2016
• 期刊: JXB.
• 作者: Nanda AK;Puyol V;Wissuwa M
• 通讯作者: Wissuwa M