Functional characterization of candidate genes for PUE and mining their allelic variants in rice

水稻 PUE 候选基因的功能表征及其等位基因变异挖掘

• 批准号: 18F18703
• 负责人: Wissuwa Matthias
• 金额: $ 1.34万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-07-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18F18703/
• 关键词: PUE; P-efficient; P-inefficient; rice genotypes; P flux; RNA sequencing; transcriptome analysis; high biomass; metabolic forms of P; fractionation of P; expression; bioinformatics; QPCR; Gene 13; Gene 14; Gene 30; PM-H+ATP; remobilization

To breed rice plants for PUE (Phosphorus use efficiency), understanding the physiological, metabolic, molecular and genetic aspects of PUE is important. In our experiments, P-efficient and P-inefficient rice genotypes were used to understand the above-mentioned traits with different doses of P supply. P-efficient genotypes maintain low concentration of P and P content and high biomass that contributed to the efficiency of the rice plant in phosphorus utilization. In addition, the P-efficient rice genotypes showed P flux from old to young leaves. In another experiment, the P-efficient genotypes showed reallocation of P from shoot to root where sampling was done at six time intervals in approx. 2 days gap.The fractionation of P into organic, nucleic acid, inorganic and metabolic components of P was studied in collaboration with Dr. Patrick Hayes (JSPS Fellow (P18711). Leaf cell-specific P-allocation was also another component looked at in this experiment. Similarly, transcriptome analysis using RNA sequencing was done for P-efficient and P-inefficient rice genotypes under different P supply. This experiment was done in collaboration with Dr. Asad Prodhan (JSPS Fellow (P19709)). These parts of the experiment will be reported by the above 2 JSPS fellows to avoid redundancy.

为了繁殖水稻植物以供PUE（磷使用效率），了解PUE的生理，代谢，分子和遗传方面很重要。在我们的实验中，使用P效率和P-粉红色的水稻基因型用于了解上述特征，并具有不同剂量的P供应。 P效率的基因型维持低浓度的P和P含量以及高生物量，这有助于大米植物在磷利用中的效率。此外，P效率的水稻基因型显示出从旧叶到幼叶的P通量。在另一个实验中，P效率的基因型显示了P从射击到根的重新分配，其中六个时间间隔进行了抽样。 2 days gap.The fractionation of P into organic, nucleic acid, inorganic and metabolic components of P was studied in collaboration with Dr. Patrick Hayes (JSPS Fellow (P18711). Leaf cell-specific P-allocation was also another component looked at in this experiment. Similarly, transcriptome analysis using RNA sequencing was done for P-efficient and P-inefficient rice genotypes under different P supply. This实验是与Asad Prodhan博士合作（JSPS研究员（P19709））。