PFI-TT: A Rapid Multiplexed Diagnostic Tool for Serology of Tick-Borne Diseases

PFI-TT：蜱传疾病血清学快速多重诊断工具

• 批准号: 2345816
• 负责人: Aydogan Ozcan
• 金额: $ 55万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-03-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2345816&HistoricalAwards=false
• 关键词: PFI; TT; Rapid; Multiplexed; Diagnostic

This Partnerships for Innovation - Technology Translation (PFI-TT) project seeks to improve blood-based detection of tick-borne diseases (TBDs) through an innovative, multiplexed, serological point-of-care (POC) assay. Addressing a critical gap in current TBD diagnostic methods, this project will develop a comprehensive platform for Lyme disease, Babesia, Anaplasma, and relapsing fever. By encompassing different TBDs co-located in similar geographical areas, the platform provides a single test solution to determine exposure to multiple TBDs, thus capturing a significant segment of the available market. The simplicity and affordability of the tool make it beneficial for resource-limited and rural areas, most impacted by TBDs. Furthermore, the platform is expected to have significant societal and healthcare benefits, allowing patients and physicians to conduct timely testing to measure immune response against multiple TBDs, which is critical in determining patient care and treatment regimens. The commercial potential of this project lies in filling a substantial market need for comprehensive and cost-effective TBD diagnostics, promising widespread adoption in various healthcare settings, including clinics and field-stations. By enhancing scientific understanding and technology in the medical diagnostics field, this project aligns with the broader goal of improving global health.This project focuses on the development of a POC, multiplexed, serological assay capable of detecting multiple TBDs simultaneously. The technology addresses the challenge of cross-reactivity, co-infection of different TBDs in endemic regions and limited sensitivity and specificity in current serological tests. By integrating specific antigen epitopes for various TBDs into a single multiplexed assay and employing advanced machine learning algorithms, this solution aims to significantly enhance serodiagnostic accuracy. The research involves iterative epitope selection and optimization of the paper-based immunoreaction platform comprising the multiplexed panel. The technology also involves developing a robust, machine learning-based diagnostic algorithm to analyze different biomarker-specific signals into interpretable and actionable diagnoses that can be used by physicians to treat patients. This project addresses the current lack of a point-of-care (POC) multiplexed test for TBDs, where patients typically require multiple tests at different centralized labs. The project's anticipated outcomes include improved serodiagnostic capabilities for various TBDs, contributing significantly to the fields of medical diagnostics and public health, particularly in areas with a high prevalence of tick-borne infections.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这种创新的合作伙伴关系 - 技术翻译（PFI-TT）项目旨在通过创新的，多重的，血清学点（POC）测定法改善基于血液的tick传播疾病（TBD）的检测。解决当前TBD诊断方法的关键差距，该项目将为莱姆病，贝贝斯，厌食和发烧开发一个全面的平台。通过涵盖在相似地理区域共同列为同期的不同TBD，该平台提供了一个单个测试解决方案，以确定暴露于多个TBD，从而捕获了可用市场的很大一部分。该工具的简单性和负担能力使其对受TBD的影响最大，对资源有限和农村地区有益。此外，该平台有望具有重大的社会和医疗保健益处，使患者和医生可以及时进行测试以测量针对多个TBD的免疫反应，这对于确定患者护理和治疗方案至关重要。该项目的商业潜力在于满足对全面且具有成本效益的TBD诊断的巨大市场需求，这有望在包括诊所和现场站在内的各种医疗机构中广泛采用。通过增强医学诊断领域的科学理解和技术，该项目与改善全球健康的更广泛的目标相吻合。该项目着重于开发POC，多重的，血清学测定能够同时检测多个TBD的血清学测定。该技术解决了交叉反应性的挑战，在地方性地区的不同TBD共同感染以及在当前血清学测试中的敏感性和特异性有限。通过将各种TBD的特定抗原表位整合到单个多路复用测定中并采用先进的机器学习算法，该解决方案旨在显着提高血清诊断的准确性。该研究涉及迭代表位选择和包括多重面板的纸基免疫反应平台的优化。该技术还涉及开发一种基于机器学习的诊断算法，以将不同的生物标志物特异性信号分析为可解释且可操作的诊断，医生可以用来治疗患者。该项目解决了目前缺乏对TBD的保健点（POC）多路复用测试，在该测试中，患者通常需要在不同的集中式实验室进行多次测试。该项目的预期结果包括提高各种TBD的血清诊断能力，对医学诊断和公共卫生的领域产生了很大的贡献，尤其是在tick传播感染率很高的领域。这奖反映了NSF的立法任务，并被认为是通过基金会的智力效果和广泛的评估来评估的，并且值得通过评估。