RUI: Self-Assembled Interfaces: Protolipids, Asymmetry, and Energetics

RUI：自组装界面：原生脂质、不对称性和能量学

• 批准号: 2304913
• 负责人: Sunghee Lee
• 金额: $ 34.5万
• 依托单位: IONA UNIVERSITY
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-12-15 至 2027-11-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2304913&HistoricalAwards=false
• 关键词: RUI; Self; Assembled; Interfaces; Protolipids

With the support of the Macromolecular, Supramolecular and Nanochemistry Program in the Division of Chemistry, Professor Sunghee Lee and her research group in the Department of Chemistry at Iona University are undertaking studies on how living cells arrange molecular components in their membranes. These boundary layers are composed of long-tailed lipid (fatty) molecules that assemble in a double layer (a bilayer) with different kinds of lipid molecules on the inner layer relative to the outer layer. This molecular difference, or asymmetry, is vital for cell function, and yet the fundamental reasons for this arrangement are not fully known. Professor Lee and her team will explore a large array of lipid compositions of cell membranes, using cell-size water droplets that self-arrange into asymmetric bilayers, and microscopically examine the resulting physical, chemical, and mechanical properties of these cell-mimics. The goal of the project is to determine how the differing shapes and sizes of individual lipid molecules can impact cell-membrane mechanical strength, leakiness, and stability, which determine the functions and the life cycle of living cells. The project has the potential to provide insights that aid in the design of soft matter systems and functional materials, and also contribute to better understanding of biologically relevant materials. The scientific broader impacts of the proposed work relate to potential longer term implications of these studies for the production of biosensors, diagnostics, and drug screening platforms. This project will also employ research methods that will help train the next generation of globally-competitive scientists with hypothesis-driven interdisciplinary research, and is expected to include participation from members of underrepresented groups. Outreach activities are planned to extend the public engagement with this research by involving middle/high school students and teachers at minority serving institutions.Under this award, Professor Sunghee Lee and her group will impose increasing spontaneous curvature upon symmetric and asymmetric planar lipid bilayers and ascertain changes in the driving force for bilayer formation, to develop a direct experimental transducer for curvature elastic stress energy. Models of symmetric and asymmetric bilayers comprising lipids of negative curvature will be examined for their effect upon the thermodynamic driving force for bilayer formation. To quantify the effects of lipid shape, the Lee group will acquire water transport parameters across the bilayer, determine bilayer stability parameters, ascertain electrical properties, use vibrational spectroscopy for determination of acyl chain order, and perform thermotropic analyses of lipid dispersions. Dissemination of the technology will include working with collaborators to apply the techniques of asymmetric droplet interface bilayers for studies of protein function.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系的大分子，超分子和纳米化学计划的支持下，Sunghee Lee教授及其在Iona University化学系的研究小组正在研究有关活细胞如何在其膜上安排分子成分的研究。这些边界层由长尾脂质（脂肪）分子组成，这些分子与外层相对于外层的双层（双层）组合（双层）。这种分子差异或不对称性对于细胞功能至关重要，但是这种布置的基本原因尚不完全清楚。 Lee教授和她的团队将使用细胞大小的水滴来探索大量细胞膜的脂质组成，这些水滴会自偏成非对称双层，并显微镜检查这些细胞模拟物的物理，化学和机械性能。该项目的目的是确定单个脂质分子的不同形状和大小如何影响细胞膜机械强度，泄漏和稳定性，从而决定活细胞的功能和生命周期。该项目有可能提供洞察力，以帮助设计软件系统和功能材料的设计，并有助于更好地理解生物学相关的材料。 拟议工作的科学广泛影响与这些研究对生物传感器，诊断和药物筛查平台的生产的潜在长期影响有关。该项目还将采用研究方法，以通过假设驱动的跨学科研究来帮助培训下一代全球竞争力的科学家，并有望包括代表性不足的群体的参与。 计划通过参与少数派服务机构的中/高中生和老师来扩展公众参与这项研究。在此奖项之后，Sunghee Lee教授和她的小组将在对称和不对称的平面脂质双层双层型双层型和驱动力的驱动器中的变化方面施加自发性的曲率，以开发Bilayer形成的横向实验。将检查包含负曲率脂质的对称和不对称双层模型，以检查其对双层形成的热力学驱动力的影响。为了量化脂质形状的影响，Lee组将获取跨双层的水传输参数，确定双层稳定性参数，确定电气性能，使用振动光谱来确定酰基链序，并执行脂质分散体的热旋转分析。该技术的传播将包括与合作者合作，应用非对称液滴接口双层蛋白功能研究的技术。该奖项反映了NSF的法定任务，并认为值得通过基金会的知识分子优点和更广泛的影响来通过评估来获得支持。

项目成果

Differential Effects of Soy Isoflavones on the Biophysical Properties of Model Membranes

• DOI: 10.1021/acs.jpcb.3c08390
• 发表时间: 2024-02-28
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY B
• 影响因子: 3.3
• 作者: Gudyka，Jamie;Ceja-Vega，Jasmin;Lee，Sunghee
• 通讯作者: Lee，Sunghee