RUI: Investigations of Pattern Formation and Morphology in Two Dimensional Self-Assembled Model Biomembranes

RUI：二维自组装模型生物膜中图案形成和形态学的研究

• 批准号: 1207544
• 负责人: Benjamin Stottrup
• 金额: $ 15.79万
• 依托单位: Augsburg University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1207544&HistoricalAwards=false
• 关键词: RUI; Investigations; Pattern; Formation; Morphology

ID: MPS/DMR/BMAT(7623) 1207544 PI: Stottrup, Benjamin ORG: Augsburg CollegeTitle: RUI: Investigations of Pattern Formation and Morphology in Two Dimensional Self-Assembled Model BiomembranesINTELLECTUAL MERIT: The complex and multi-component plasma cell membrane provides inspiration for advances in nanotechnology and the development of new materials. In the past decade, consensus has grown around the importance of cholesterol for supramolecular lateral organization within this system. Today, cholesterol is recognized as crucial in the lateral organization of these membranes in both in vitro (lipid monolayers and bilayers) and in vivo (plasma cell membrane) systems. In Langmuir monolayers containing cholesterol and phospholipids the observed phase separation has been shown to be the result of a competition between long-range electrostatic forces and line tension in two dimensions. However, important questions remain regarding the true equilibrium nature of these systems, the size distribution of their domains, and the role of cholesterol's molecular structure in the observed phase separation. This project will test the hypothesis that the morphology of phase separated lipid monolayers containing cholesterol can be controlled by transition kinetics, monolayer composition, and sterol structure to enable the patterning of solid substrates. Motivation to control lipid membrane patterning comes from the applications of self-assembled lipid systems to the fabrication of biosensors and other biomaterials. Lipid monolayers are regularly used in the fabrication of supported lipid bilayers on solid substrates. Additionally, this project will explore the use of phase separated lipid monolayers to organize nanoparticles at the air-water interface. Through collaborations and partnerships this project will extend recently developed techniques in model convolution microscopy, new line tension measurement methods, and incorporate a novel micro-fluidic flow cell into a traditional fluorescence microscopy set-up. BROADER IMPACTS: The scientific objectives of this project will be achieved within the context of research at a primarily undergraduate institution. Providing high quality research experiences to train and encourage undergraduates from diverse backgrounds to pursue careers in science, technology, engineering, and mathematics has been a central component of the principal investigator's role as an educator and mentor over the past seven years. Consistent with the demographics of Augsburg College, the principal investigator will continue to encourage and recruit participation from NSF-defined underrepresented populations. In addition to close faculty interactions through research, this project will provide students with opportunities to develop their understanding of vocational and career objectives through regular contact with collaborators and practicing scientists. The principal investigator will leverage Augsburg's Office of Undergraduate Research and Graduate Opportunities (URGO) to supplement these experiences through professional training as well as assistance in the application process for fellowships and graduate school. Weekly group meetings provide students with opportunities to build communication skills and confidence. Students will participate in the dissemination of results through presentations at regional and national conferences as well as co-authorship of on peer-reviewed publications. This project will also enrich the undergraduate curriculum at Augsburg College. Fluorescence microscopy and image analysis techniques used in this research will be incorporated into undergraduate laboratory experiences for physics majors in sophomore and senior level courses.

ID：MPS/DMR/BMAT(7623) 1207544 PI：Stottrup，Benjamin ORG：奥格斯堡学院标题：RUI：二维自组装模型生物膜中的图案形成和形态学研究智力优点：复杂且多组分的浆细胞膜提供了灵感表彰纳米技术的进步和新材料的开发。 在过去的十年中，关于胆固醇对于该系统内超分子横向组织的重要性已经达成共识。 如今，胆固醇被认为在体外（脂质单层和双层）和体内（浆细胞膜）系统中这些膜的横向组织中至关重要。 在含有胆固醇和磷脂的朗缪尔单分子层中，观察到的相分离已被证明是长程静电力和二维线张力之间竞争的结果。 然而，关于这些系统的真正平衡性质、其域的大小分布以及胆固醇分子结构在观察到的相分离中的作用仍然存在重要问题。 该项目将测试以下假设：含有胆固醇的相分离脂质单层的形态可以通过转变动力学、单层组成和甾醇结构来控制，从而实现固体基质的图案化。 控制脂质膜图案的动机来自于自组装脂质系统在生物传感器和其他生物材料制造中的应用。 脂质单层通常用于在固体基质上制造支撑脂质双层。 此外，该项目将探索使用相分离的脂质单层在空气-水界面组织纳米颗粒。 通过合作和伙伴关系，该项目将扩展最近开发的模型卷积显微镜技术、新的线张力测量方法，并将新型微流体流动池纳入传统的荧光显微镜装置中。更广泛的影响：该项目的科学目标将在主要本科机构的研究背景下实现。 过去七年里，首席研究员作为教育者和导师的核心职责是提供高质量的研究经验，培训和鼓励来自不同背景的本科生从事科学、技术、工程和数学领域的职业。 与奥格斯堡学院的人口统计数据一致，首席研究员将继续鼓励和招募 NSF 定义的代表性不足人群的参与。 除了通过研究进行密切的教师互动外，该项目还将为学生提供通过与合作者和实践科学家定期接触来加深对职业和职业目标的理解的机会。 首席研究员将利用奥格斯堡的本科生研究和研究生机会办公室（URGO），通过专业培训以及在奖学金和研究生院申请过程中的协助来补充这些经验。 每周的小组会议为学生提供了培养沟通技巧和信心的机会。 学生将通过在地区和国家会议上的演讲以及共同撰写同行评审出版物来参与成果的传播。 该项目还将丰富奥格斯堡学院的本科课程。 本研究中使用的荧光显微镜和图像分析技术将被纳入物理专业大二和高年级课程的本科生实验室经验中。