RUI: Structural Determinants of Permeation and Nucleation at a Self-Assembled Interface

RUI：自组装界面渗透和成核的结构决定因素

• 批准号: 1609135
• 负责人: Sunghee Lee
• 金额: $ 32.5万
• 依托单位: IONA UNIVERSITY
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1609135&HistoricalAwards=false
• 关键词: RUI; Structural; Determinants; Permeation; Nucleation

In this research program, with support from the Macromolecular, Supramolecular and Nanochemistry Program of the Division of Chemistry, Professor Sunghee Lee of Iona College and her undergraduate students gain greater fundamental understanding of how living cells maintain their water balance - a property that is essential to life. They use nature-inspired techniques to create tailor-made particles - these particles are cell-sized water droplets which are used as models for natural cellular systems. A large number of undergraduate students are engaged in this research and many of these students publish peer-reviewed manuscripts. Diverse outreach seminars and science symposia involving high school students and teachers are conducted to inspire and encourage the next generation of STEM educators. Professor Lee reaches out to underrepresented groups in particular. In addition to understanding the water balance in living cells, the research could also have industrial applications in the design of reverse osmosis systems used to purify water.This project focuses on understanding how supramolecular aggregates assembled at liquid-liquid interfaces (including surfactant bilayers) mediate the passage of small molecules and direct biomineralization. Systematic studies determine the effect of bilayer-incorporated materials (e.g., nanomaterials) upon the characteristics of water transport through the droplet interface bilayer (DIB). Experimental activities primarily focus on acquisition of water permeability parameters for osmotic flow in conjunction with the determination of electrical properties, and compositional studies via Raman quantification. Experimental strategies provide insight into how bilayered supramolecular assemblies promote the crystallization of important targets (biominerals and proteins), through nucleation studies of biomineral formation. Biomineralization is the process by which living organisms produce minerals that are often used to harden or stiffen existing tissues.

在该研究计划中，在化学系的大分子，超分子和纳米化学计划的支持下，Iona College的Sunghee Lee教授及其本科生对生活细胞如何维持水平的水平平衡获得了更大的基本了解 - 一种对生活至关重要的财产。他们使用自然风格的技术来创建量身定制的颗粒 - 这些颗粒是细胞大小的水滴，用作天然细胞系统的模型。大量的本科生从事这项研究，其中许多学生出版了经过同行评审的手稿。涉及高中生和老师的各种宣传研讨会和科学研讨会是为了激发和鼓励下一代的STEM教育者。李教授尤其与代表性不足的群体接触。除了了解活细胞的水平平衡外，该研究还可以在用于净化水的反渗透系统的设计中具有工业应用。本项目着重于了解如何在液 - 液体界面（包括表面活性剂双层）组装的超分子聚集体（包括介导小分子和直接生物促进生物水中）。系统研究确定了均匀的材料（例如纳米材料）对通过液滴界面双层（DIB）水传输的特征的影响。实验活性主要集中于以渗透流量的水为结合的水渗透率参数，并通过拉曼定量确定电性能和组成研究。 实验策略提供了关于双层超分子组件如何通过生物矿物形成的成核研究研究的重构超分子组件（生物矿物和蛋白质）的结晶。 生物矿化是生物生物产生通常用于硬化或加强现有组织的矿物质的过程。

项目成果

Ibuprofen and the Phosphatidylcholine Bilayer: Membrane Water Permeability in the Presence and Absence of Cholesterol

布洛芬和磷脂酰胆碱双层：存在和不存在胆固醇时的膜水渗透性

• DOI: 10.1021/acs.langmuir.0c03638
• 发表时间: 2021
• 期刊: Langmuir
• 影响因子: 3.9
• 作者: Wood, Megan;Morales, Michael;Miller, Elizabeth;Braziel, Samuel;Giancaspro, Joseph;Scollan, Patrick;Rosario, Juan;Gayapa, Alyssa;Krmic, Michael;Lee, Sunghee
• 通讯作者: Lee, Sunghee