EDGE FGT: Development of fibroblasts and organoids as tools for functional genomics in turtles, applicable to other non-mammalian vertebrates

EDGE FGT：开发成纤维细胞和类器官作为海龟功能基因组学工具，适用于其他非哺乳动物脊椎动物

基本信息

批准号：
2127995
负责人：
Nicole Valenzuela
金额：
$ 130万
依托单位：
Iowa State University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2127995&HistoricalAwards=false
关键词：
EDGE FGT Development fibroblasts organoids

项目摘要

This project at Iowa State University will develop genomic tools for turtles to advance the use of these non-model taxa (and other reptiles) to study genome-to-phenome mapping, gene function, development, genome architecture, adaptive responses to climate change, and more. Turtles are an emerging model for ecology, evolution, and other topics, but genomic tools are needed for that promise to be fully realized. This project will provide the scientific community with a biobank and tools applicable to turtles and other reptiles. Workshops, symposia, and publications will enable the rapid dissemination of these resources. Training will be provided across levels of career development (undergraduate, graduate, postdoctoral, and faculty), including individuals from underrepresented groups. Public outreach activities will be developed to engage children and adults at the Science Center of Iowa to understand turtle biology and general science. Functional genomic resources will be developed in painted turtle fibroblasts and organoids, including gene upregulation (lentiviral vectors or RNA injection), gene knockdown (RNAi), and targeted mutagenesis tools (CRISPR-Cas9). The tools will further enable turtles as model systems to illuminate gene regulation, the underpinnings of vertebrate development, and the effects of structural variation on genome function by enabling tests of genome-to-phenome mapping. Using fibroblasts and organoids also solves the bottleneck from the seasonality of eggs for experimentation and functional genomic approaches with transient transgenics mitigates some of the challenge of studying long-lived organisms. Developing fibroblasts and organoids as new systems for functional genomics will catalyze the study of climate change effects in turtles and other reptiles.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

爱荷华州立大学的该项目将开发乌龟的基因组工具，以推动使用这些非模型分类单元（和其他爬行动物）来研究基因组到晶状体映射，基因功能，发展，基因组建筑，对气候变化的适应性反应等等。乌龟是生态学，进化和其他主题的新兴模型，但是为了充分实现该承诺，需要基因组工具。该项目将为科学界提供适用于海龟和其他爬行动物的生物库和工具。研讨会，研讨会和出版物将使这些资源的快速传播。包括来自代表性不足的团体的个人，包括职业发展水平（本科，研究生，博士后和教职员工）的培训。将开发公共外展活动，以吸引爱荷华州科学中心的儿童和成人，以了解乌龟生物学和一般科学。功能性基因组资源将在涂有龟的成纤维细胞和类器官中开发，包括基因上调（慢病毒载体或RNA注射），基因敲低（RNAI）和靶向诱变工具（CRISPR-CAS9）。这些工具将进一步使乌龟成为模型系统，以阐明基因调节，脊椎动物发育的基础以及结构变异对基因组功能的影响，通过启用基因组到晶状体映射的测试。使用成纤维细胞和类器官还解决了卵子的季节性的瓶颈，用于实验和功能性基因组方法，其瞬时转基因可以减轻研究长寿命生物体的挑战。开发成纤维细胞和器官作为功能基因组学的新系统将催化乌龟和其他爬行动物的气候变化影响的研究。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响标准通过评估来进行评估的。